| 绿色木霉固态发酵生产纤维素酶条件优化与酶的固定化 |
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| 引用本文： | 王晓明,孙玉辉,张欢,刘齐,董晓莹,曹焱鑫,邵丽杰,张大雷,寇巍.绿色木霉固态发酵生产纤维素酶条件优化与酶的固定化[J].浙江农业学报,2014,26(1):0. |
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| 作者姓名： | 王晓明 孙玉辉 张欢 刘齐 董晓莹 曹焱鑫 邵丽杰 张大雷 寇巍 |
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| 作者单位： | 辽宁省能源研究所，辽宁 营口 115003 |
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| 基金项目： | 国家高技术研究发展计划(863计划)(2009AA10Z407) |
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| 摘 要： | 以麸皮与秸秆粉为主要原料，对影响绿色木霉固态发酵的因素如麸皮与秸秆粉的比例、发酵温度、时间、含水率、初始pH值、氮源浓度等进行研究。在单因素实验的基础上，采取正交实验设计，结果表明最佳固体发酵条件为：麸皮与秸秆比例4∶1，含氮量2%，培养温度28℃，培养时间72 h，起始pH 5，接种量10%，含水率175%（相对于固体发酵底物）。在此条件下，CMC 酶活达到1 1325 U·g-1，比未优化条件下酶活6124 U·g-1，提高了849%。利用包埋、交联和交联包埋三种方法对纤维素酶进行固定化，其中交联包埋法效果较好。
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| 关 键 词： | 绿色木霉 纤维素酶 正交试验设计 固定化纤维素酶 |
Studies on the conditions of solid\|state fermentation for cellulase production by Trichoderma viride and cellulase immobilization |
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| WANG Xiao\|ming,SUN Yyu\|hui,ZHANG Huan,LIU Qi,DONG Xiao\|ying,CAO Yan\|xin,SHAO Li\|jie,ZHANG Da\|lei,KOU Wei.Studies on the conditions of solid\|state fermentation for cellulase production by Trichoderma viride and cellulase immobilization[J].Acta Agriculturae Zhejiangensis,2014,26(1):0. |
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| Authors: | WANG Xiao\|ming SUN Yyu\|hui ZHANG Huan LIU Qi DONG Xiao\|ying CAO Yan\|xin SHAO Li\|jie ZHANG Da\|lei KOU Wei |
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| Institution: | Liaoning Institute of Energy Resource， Yingkou 115003， China |
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| Abstract: | Using wheat bran and corn straw as raw materials， the influence of the ratio of wheat bran to corn straw， fermentation temperature and time， moisture content， initial pH value， nitrogen concentration， on cellulose production by solid\|state fermentation of Trichoderma viride were studied in this paper. Based on optimization of solid\|state fermentation by single\|factor test， orthogonal test was made. The results showed that the optimal solid\|state fermentation conditions for cellulase were listed as follows：the ratio of wheat bran to corn straw powder was 4∶1，nitrogen concentration was 2%， culture temperature was 28℃， fermentation time was 72 h， initial pH value was 5， inoculum level was 10% and moisture content （based on solid fermentation substrate） was 175%. In this condition，maximum cellulase activity was 1 1325 U·g-1，which was increased 849% than 6124 U·g-1 in initial fermentation condition. Cellulase was immobilized by embedding， cross\|linking and embed\|cross\|linking method， in which embed\|cross\|linking method was the best one. |
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| Keywords: | Trichoderma viride cellulase orthogonal experimental design immobilized cellulase |
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