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宗地花猪H-FABP基因mRNA的表达与肉质性状的相关研究
引用本文:李平,燕志宏,陈伟,张芸,田松军.宗地花猪H-FABP基因mRNA的表达与肉质性状的相关研究[J].中国畜牧兽医,2015,42(3):644-649.
作者姓名:李平  燕志宏  陈伟  张芸  田松军
作者单位:1. 贵州大学动物科学学院, 贵阳 550025;
2. 高原山地动物遗传育种与繁殖省部共建教育部重点实验室, 贵阳 550025;
3. 贵州省畜禽遗传资源管理站, 贵阳 550001;
4. 紫云县畜牧兽医局, 紫云 561000
基金项目:贵州省科技厅农业攻关项目(黔科合NZ字[2012]3013号);紫云宗地花猪生态养殖技术集成与产业化示范[黔科合县市科拨计划(2012)7032]
摘    要:为研究不同饲养条件对地方猪种宗地花猪心脏型脂肪酸结合蛋白(heart fatty acid binding protein,H-FABP)基因表达及肌内脂肪(intramuscular fat,IMF)含量的影响,试验利用实时荧光定量PCR技术,分别测定放养型和圈养型宗地花猪背最长肌中H-FABP基因的表达水平,并测定背最长肌的IMF、大理石纹、嫩度.结果发现,圈养型宗地花猪的IMF、大理石纹、嫩度均高于放养型宗地花猪(P >0.05),pH、肉色极显著高于放养型宗地花猪(P <0.01);放养型宗地花猪背最长肌中H-FABP mRNA 水平显著高于圈养型宗地花猪(P <0.05);放养型、圈养型宗地花猪H-FABP基因表达与IMF均无显著相关性(P >0.05).以上结果表明,不同饲养条件影响H-FABP基因表达并在一定程度上影响IMF,但影响不显著.

关 键 词:宗地花猪  H-FABP基因  肌内脂肪  
收稿时间:2014-09-09

Association Analysis on H-FABP Gene Expression Level and Meat Quality Traits of Zongdi Spotted Pigs
LI Ping;YAN Zhi-hong;CHEN Wei;ZHANG Yun;TIAN Song-jun.Association Analysis on H-FABP Gene Expression Level and Meat Quality Traits of Zongdi Spotted Pigs[J].China Animal Husbandry & Veterinary Medicine,2015,42(3):644-649.
Authors:LI Ping;YAN Zhi-hong;CHEN Wei;ZHANG Yun;TIAN Song-jun
Institution:1. College of Animal Science, Guizhou University, Guiyang 550025, China;
2. Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guizhou University, Guiyang 550025, China;
3. The Management Station of Livestock and Poultry Genetic Resources in Guizhou Province, Guiyang 550001, China;
4. The Animal Husbandry and Veterinary in Ziyun County, Ziyun 561000, China
Abstract:Different feeding conditions of Zongdi spotted pigs,H-FABP gene expression level was detected in back fat by Real-time PCR and IMF content in longissimus muscle of back was determined.The results showed that IMF,marble and tenderness of captivity type Zongdi spotted pigs were higher than grazing type (P >0.05),pH and meat color of captivity type Zongdi spotted pigs were very significantly higher than grazing type (P <0.01); H-FABP mRNA expression level of grazing type longissimus muscle of back was significantly higher than captivity type Zongdi spotted pigs (P <0.05);There was no significant correlation between H-FABP mRNA expression level and IMF content partly in grazing type and captivity type (P >0.05).Different feeding conditions could influence H-FABP mRNA expression and IMF content partly,yet influence was not significantly.
Keywords:Zongdi spotted pigs  H-FABP gene  IMF
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