| 状元红葡萄的脱毒与快繁技术研究 |
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| 引用本文： | 杨光,金桂花,董俊,张青,龚娜.状元红葡萄的脱毒与快繁技术研究[J].浙江农业学报,2014,26(1):0. |
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| 作者姓名： | 杨光 金桂花 董俊 张青 龚娜 |
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| 作者单位： | 辽宁省农业科学院 园艺分院， 辽宁 沈阳 110161 |
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| 摘 要： | 以状元红葡萄为材料，选取秋季副梢的茎尖为外植体， 通过直接诱导丛生芽进行快速繁殖，同时运用热处理和茎尖培养相结合诱导葡萄脱毒苗。脱毒技术研究结果表明，1/2MS培养基比MS培养基的芽诱导率高。适宜的启动培养基为1/2MS+20 mg·L-1 6\|BA+01 mg·L-1 IBA，诱导效果最好。无根苗接种于1/2MS+05 mg·L-1 IBA+02 mg·L-1 NAA的培养基中，生根快，不定根发育良好，利于移栽。其中热处理时间以10 d为宜，这样有利于无根苗的生长和脱毒；经过热处理的无根苗，脱毒率达100%。
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| 关 键 词： | 葡萄 茎尖 脱毒 快繁 |
Study on virus\|free and rapid propagation techniques of Zhuangyuanhong grape |
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| YANG Guang,JIN Gui\|hua,DONG Jun,ZHANG Qing,GONG Na.Study on virus\|free and rapid propagation techniques of Zhuangyuanhong grape[J].Acta Agriculturae Zhejiangensis,2014,26(1):0. |
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| Authors: | YANG Guang JIN Gui\|hua DONG Jun ZHANG Qing GONG Na |
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| Institution: | Research Horticulture Branch, Liaoning Academy of Agricultural Sciences， Shenyang 110161， China |
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| Abstract: | Zhuangyuanhong grape was used as experimental material， the shoot tips of stem apexes were selected as explants， which reproduced rapidly through directly inducing cluster buds， then the methods of heat treatment and tip culture were combined to induce virus\|free grape. The results showed that shoots induction rate in medium 1/2MS was higher than MS. The best inducing effects was found in the medium of 1/2 MS+20 mg·L-1 6\|BA+01 mg·L-1 IBA. Rootless plantlets inoculated in the medium of 1/2MS+05 mg·L-1 IBA+02 mg·L-1 NAA grew quickly and the growth of adventitious root was good， which were ready for transplanting. The optimal heat treatment time was 10 days which was beneficial for growth and virus elimination of rootless plantlets. After the heat treatment， 100% target viruses in the rootless plantlets were taken off. |
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| Keywords: | grape shoot tip virus elimination rapid propagation |
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