| 利用 SSR 标记分析高粱属种质资源的遗传多样性 |
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| 引用本文： | 王芳,高秋,王杰,马金星,孙娟.利用 SSR 标记分析高粱属种质资源的遗传多样性[J].草业学报,2016,25(5):125-133. |
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| 作者姓名： | 王芳 高秋 王杰 马金星 孙娟 |
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| 作者单位： | 青岛农业大学经济草本植物应用研究所,山东 青岛,266109;农业部全国草产品质量监督检验测试中心,北京,100125 |
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| 基金项目： | 国家牧草产业技术体系项目资助。 |
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| 摘 要： | 本研究利用15对SSR引物对161份高粱属种质资源进行了遗传多样性分析。结果显示,15对引物共扩增出118条谱带,其中88条具有多态性,多态性比率(P)为75.21%;多态性信息含量(PIC值)变幅为0.612~0.806,平均为0.699。161份高粱属材料遗传相似性系数为0.636~0.977,平均基因多样性指数(H)为0.696,Shannon信息指数(I)为1.393。UPGMA聚类分析显示,161份高粱属材料在遗传相似系数(GS)为0.682处可分为3组;129份高粱和苏丹草材料在相似系数为0.671处可分为2组。表明SSR标记可以作为高粱属种间以及种内遗传分化分析的有力工具,被分析的高粱属种质材料具有较高的遗传多样性。
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| 关 键 词： | 高粱属 种质资源 SSR 遗传多样性 |
| 收稿时间： | 2015-07-07 |
Analysis of genetic diversity in Sorghum germplasm collections using SSR markers |
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| WANG Fang,GAO Qiu,WANG Jie,MA Jin-Xing,SUN Juan.Analysis of genetic diversity in Sorghum germplasm collections using SSR markers[J].Acta Prataculturae Sinica,2016,25(5):125-133. |
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| Authors: | WANG Fang GAO Qiu WANG Jie MA Jin-Xing SUN Juan |
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| Abstract: | Fifteen SSR markers were used to quantify the genetic diversity of the core germplasm collections of sorghum.A total of 161 accessions were assayed.118 bands were generated,among which 88 (75.21%)were polymorphic.The average value of polymorphic information content (PIC)was 0.699 (0.612-0.806);the av-erage Nei’s gene diversity index (H)was 0.696;and the average Shannon information index (I)was 1.393. The genetic similarities among the 161 accessions ranged from 0.636 to 0.977.The UPGMA dendrogram indi-cated that the 161 Sorghum germplasm collections were clustered into three groups with a threshold of 0.682, and that the 129 Sorghum sudanense and Sorghum bicolorx ×Sorghum sudanense germplasm resources were di-vided into two clusters at a threshold of 0.671.This study demonstrated that there is considerable genetic di-versity conserved in Sorghum germplasm collections and SSR is an ideal molecular marker for assessing this di-versity. |
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| Keywords: | Sorghum germplasm resource simple sequence repeat (SSR) genetic diversity |
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