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| 玉米和水稻全基因组控制重组频率QTL的遗传定位分析 | |
| 引用本文： | 李林,李青,王立博,张祖新,李建生,严建兵.玉米和水稻全基因组控制重组频率QTL的遗传定位分析[J].中国农业科学,2009,42(7):2262-2270. |
| 作者姓名： | 李林 李青 王立博 张祖新 李建生 严建兵 |
| 作者单位： | 1. 中国农业大学国家玉米改良中心,北京,100193,中国 2. 河北农业大学农学院,河北保定,071001,中国 3. 中国农业大学国家玉米改良中心,北京,100193,中国;国际小麦玉米改良中心(CIMMYT),Apdo.Postal 6-641,06600,Mexico,D.F,墨西哥 |
| 基金项目： | 国家自然科学基金，国家高技术研究发展计划(863计划) |
| 摘 要： | 【目的】尝试利用分子标记和QTL定位技术直接定位影响重组频率的QTL，探索物种遗传变异难易的分子基础。【方法】分别借助3张玉米和3张水稻分子标记连锁图，以所有染色体上标记的交换次数为性状进行分析。【结果】分别定位了7个和11个影响玉米和水稻重组频率的QTL。以玉米和水稻高密度分子标记连锁图IBM302和Genetic98每条染色体上标记的交换次数为性状，分别在玉米和水稻上定位了12个和57个QTL。【结论】影响重组频率的基因真实存在。培育高重组频率材料将有助于加速基因的定位和克隆、比较图位克隆基因和遗传育种进程。 |
| 关 键 词： | |
| 收稿时间： | 2008-09-23; |
Genetic Analysis of QTL Affecting Recombination Frequency in Whole Genome of Maize and Rice |
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| LI Lin,LI Qing,WANG Li-bo,ZHANG Zu-xin,LI Jian-sheng,YAN Jian-bing.Genetic Analysis of QTL Affecting Recombination Frequency in Whole Genome of Maize and Rice[J].Scientia Agricultura Sinica,2009,42(7):2262-2270. | |
| Authors: | LI Lin LI Qing WANG Li-bo ZHANG Zu-xin LI Jian-sheng YAN Jian-bing |
| Institution: | (National Maize Improvement Center of China, China Agricultural University) |
| Abstract: | 【Objective】 Recombination plays an important role in species evolution as well as plant and animal breeding. Here, the number of crossover breakpoints was used as a trait to map the QTL controlling recombination of genomes. 【Method】 Total number of crossover breakpoints across all chromosomes (NCAC) was used as the trait. 【Result】 Seven and eleven QTL were detected in 3 maize and 3 rice genetic populations, respectively. Meanwhile, 12 and 57 more QTL were detected in the maize IBM302 and rice Genetic98 population, respectively, when using the number of crossover breakpoints per chromosome (NCPC) as the trait. The number of QTL detected is strongly correlated with the resolution of the genetic linkage map. 【Conclusion】 These results not only have provided evidences for the true existence of genes controlling recombination frequency, but also facilitate our further research on map-based gene cloning and molecular breeding. |
| Keywords: | quantitative trait loci recombination frequency molecular breeding map-based gene cloning |
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