| 普通小麦品种“豫麦66”抗白粉病基因的鉴定与分子标记 |
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| 引用本文： | 胡铁柱,李洪杰,刘子记,解超杰,周益林,段霞瑜,贾旭,尤明山,杨作民,孙其信,刘志勇.普通小麦品种“豫麦66”抗白粉病基因的鉴定与分子标记[J].作物学报,2008,34(4):545-550. |
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| 作者姓名： | 胡铁柱 李洪杰 刘子记 解超杰 周益林 段霞瑜 贾旭 尤明山 杨作民 孙其信 刘志勇 |
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| 作者单位： | 1.中国农业大学农业生物技术国家重点实验室 / 农业部作物基因组学与遗传改良重点开放实验室 / 北京市作物遗传改良重点实验室 /教育部作物杂种优势研究与利用重点实验室, 北京100094;2.河南科技学院小麦中心, 河南新乡453003;3.国家农作物遗传资源与基因改良重大科学工程 / 中国农业科学院作物科学研究所, 北京100081;4.中国农业科学院植物保护研究所 / 植物病虫害生物学国家重点实验室, 北京100094;5.中国科学院遗传与发育生物学研究所 / 植物细胞与染色体工程国家重点实验室, 北京100101 |
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| 基金项目： | 国家高技术研究发展计划(863计划)
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国家自然科学基金
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教育部长江学者和创新团队发展计划
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高等学校学科创新引智计划项目 |
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| 摘 要： | 豫麦66是对小麦白粉病(Blumeria graminis f. sp. tritici)具有良好抗性的小黑麦后代品种。本试验通过抗病鉴定与遗传分析, 明确了豫麦66携带1个抗白粉病显性单基因, 暂命名为PmYm66。采用2个以豫麦66为抗病亲本的杂交组合(豫麦66/铭贤169和豫麦66/ND3509)F2代抗、感病分离群体和F3代家系, 利用集群分离分析法(BSA)找到了与PmYm66连锁的分子标记XKsum193、EST48、EST83和EST84, 抗病基因和分子标记的顺序为EST48—EST83 (EST84)—Xksum193—PmYm66, 并通过中国春缺体-四体、双端体和缺失系将PmYm66基因及其连锁的分子标记定位在2AL染色体臂末端。多小种鉴定结果表明PmYm66(豫麦66)与2AL染色体臂上已有的Pm4a(Khapli/8Cc)和Pm4b(Armada)基因存在致病反应型差异。
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| 关 键 词： | 小麦 豫麦66 白粉病 抗病基因 分子标记 |
| 文章编号： | 10.3724/SP.J.1006.2008.00545 |
| 收稿时间： | 2007-11-07 |
| 修稿时间： | 2007年11月7日 |
Identification and Molecular Mapping of the Powdery Mildew Resistance Gene in Wheat Cultivar Yumai 66 |
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| HU Tie-Zhu,LI Hong-Jie,LIU Zi-Ji,XIE Chao-Jie,ZHOU Yi-Lin,DUAN Xia-Yu,JIA Xu,YOU Ming-Shan,YANG Zuo-Min,SUN Qi-Xin,LIU Zhi-Yong.Identification and Molecular Mapping of the Powdery Mildew Resistance Gene in Wheat Cultivar Yumai 66[J].Acta Agronomica Sinica,2008,34(4):545-550. |
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| Authors: | HU Tie-Zhu LI Hong-Jie LIU Zi-Ji XIE Chao-Jie ZHOU Yi-Lin DUAN Xia-Yu JIA Xu YOU Ming-Shan YANG Zuo-Min SUN Qi-Xin LIU Zhi-Yong |
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| Institution: | 1 State Key Laboratory for Agrobiotechnology, Department of Plant Genetics & Breeding / Key Laboratory of Crop Genomics and Genetic Improvement, Ministry of Agriculture / Beijing Key Laboratory of Crop Genetic Improvement / Key Laboratory of Crop Heterosis Research & Utilization, Ministry of Education, China Agricultural University, Beijing 100094; 2 Wheat Center, Henan Institute of Science and Technology, Xinxiang 453003, Henan; 3 National Key Facility for Crop Gene Resources and Genetic Improvement / Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081; 4 Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094; 5 State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China |
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| Abstract: | Powdery mildew, caused by Blumeria graminis f. sp. tritici, is one of the most important diseases of wheat (Triticum aestivum L.) worldwide. Breeding for resistance is the most economical and effective method for controlling the disease. Yumai 66, a common wheat cultivar derived from a wheat-triticale cross, is resistant to a wide spectrum of wheat powdery mildew isolates from both China and Canada. In the present study, genetic analysis indicated that Yumai 66 carried a single dominant gene for resistance to powdery mildew, designated tentatively PmYm66. The segregating F2 populations and their F3 progenies of the crosses Yumai 66/Mingxian 169 and Yumai 66/ND3509 were used for bulked segregation analysis (BSA). Four molecular markers were associated with PmYm66 in an order of EST48–EST83 (EST84)–Xksum193–PmYm66. These SSR and EST markers were assigned to the distal bin of chromosome 2AL by means of Chinese Spring nullisomic-tetrasomic, ditelisomic, and deletion lines. The reaction types of Yumai 66 to 21 B. graminis f. sp. tritici isolates from China was different from that of Pm4a and Pm4b located on chromosome 2AL. |
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| Keywords: | Wheat Yumai66 Powdery mildew Resistance gene Molecular marker |
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