| 基于SSR分子标记的杜仲遗传多样性体系建立 |
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| 引用本文： | 黄海燕,杜红岩,乌云塔娜,朱高浦.基于SSR分子标记的杜仲遗传多样性体系建立[J].林业科学研究,2013,26(6):795-799. |
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| 作者姓名： | 黄海燕 杜红岩 乌云塔娜 朱高浦 |
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| 作者单位： | 中国林业科学研究院经济林研究开发中心, 河南 郑州 450003;中国林业科学研究院经济林研究开发中心, 河南 郑州 450003;国家林业局经济林育种与栽培重点实验室, 湖南 长沙 410004;中国林业科学研究院经济林研究开发中心, 河南 郑州 450003 |
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| 基金项目： | 国家林业公益性行业科研专项（201004029）、国家十二五科技支撑计划（2012BAD21B0502） |
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| 摘 要： | In order to set up the genetic diversity system of Eucommia ulmoides Oliver based on SSR molecular markers, the establishment of SSR-PCR reaction system and screening out SSR marker primer showing high polymorphism were studied. A L9(34) orthogonal design was performed to optimize the main factors of the SSR-PCR reaction system. The results indicated that the best SSR-PCR reaction system for E.ulmoides was DNA template 1 μL (30~60 ng·μL-1), 2×Taq PCR Master Mix 10 μL, primer 1 μL with the total volume of 25 μL. The PCR reaction system had high stability and repeatability, the pairs of SSR primers with high polymorphism were gotten. The 8 E.ulmoides samples' DNA sequence was amplified with 13 pairs of SSR primers by SSR-PCR technique, 34 alleles were detected, 2.6 alleles were detected from per site on average. Each allele's effective number was 1.751 5, and the h value was 0.379 8, the average I value was 0.643 3. This study is helpful in using SSR molecular marker to analyze genetic diversity and genetic relationship in E. ulmoides.
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| 关 键 词： | 杜仲 种质资源 SSR-PCR反应体系 引物筛选 |
| 收稿时间： | 2012/11/25 0:00:00 |
Establishment of Genetic Diversity System of Eucommia ulmoides Based on SSR Molecular Markers |
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| HUANG Hai-yan,DU Hong-yan,WUYUN Tana and ZHU Gao-pu.Establishment of Genetic Diversity System of Eucommia ulmoides Based on SSR Molecular Markers[J].Forest Research,2013,26(6):795-799. |
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| Authors: | HUANG Hai-yan DU Hong-yan WUYUN Tana and ZHU Gao-pu |
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| Institution: | Non-timber Forestry Research and Development Center, Chinese Academy of Forestry, Zhengzhou 450003, He'nan, China;Non-timber Forestry Research and Development Center, Chinese Academy of Forestry, Zhengzhou 450003, He'nan, China;Key Laboratory of Non-wood Forest Products of State Forestry Administration, Changsha 410004, Hu'nan, China;Non-timber Forestry Research and Development Center, Chinese Academy of Forestry, Zhengzhou 450003, He'nan, China |
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| Abstract: | |
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| Keywords: | Eucommia ulmoides Oliver germplasm resources optimization of SSR-PCR system primer screening |
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