DNA fingerprint of F1 interspecific hybrids from the Triticeae tribe using ISSRs |
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| Authors: | A Carvalho Manuela Matos José Lima-Brito H Guedes-Pinto César Benito |
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| Institution: | (1) Department of Genetics and Biotechnology, CGB/ICETA-UTAD, University of Trás-os-Montes and Alto Douro, Apartado 1013, 5000-911 Vila Real, Portugal;(2) Department of Genetics, Faculty of Biology, Complutense University, 28040 Madrid, Spain |
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| Abstract: | Inter-simple-sequence repeat (ISSR) were used to fingerprint three kinds of F1 interspecific hybrids: the Portuguese durum wheat cultivar ‘Candial’ × tritordeum, the Portuguese bread wheat cultivar ‘Barbela’ × tritordeum and the Portuguese triticale cultivar ‘Douro’ × tritordeum. Among the 30 primers tested, the number of discriminative primers varies from 13 to 20 in each hybrid combination, the total number of ISSR loci analysed ranged from 101 to 183, and the number of discriminative ISSR loci varied from 50 to 71. ISSR-PCR technique revealed to be a valuable fingerprint system for all F1 hybrid combinations and respective parents analysed here. The complementary use of molecular cytogenetic techniques and molecular markers could allow a more accurately evaluation and characterisation of different plant species. |
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| Keywords: | DNA fingerprint interspecific hybrids ISSR Triticeae |
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