| 龙葵果提取物抗氧化活性测定及酚类活性成分分析 |
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| 引用本文： | 腾飞,袁长鹏,王萍.龙葵果提取物抗氧化活性测定及酚类活性成分分析[J].安徽农业科学,2014(19):6217-6219. |
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| 作者姓名： | 腾飞 袁长鹏 王萍 |
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| 作者单位： | 东北林业大学林学院; |
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| 基金项目： | 国家自然科学基金(31170510) |
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| 摘 要： | 目的]比较不同龙葵果提取物的抗氧化活性,同时测定其活性成分.方法]用浓度60％乙醇浸提龙葵果,获得粗提物,减压蒸馏后分别用正丁醇、乙酸乙酯、氯仿3种溶剂萃取,采用DPPH·、·OH,O2-·清除法对各提取物的抗氧化作用进行评价,并与浓度60％乙醇粗提物溶液进行比较;分别测定4种龙葵果提取物中酚酸、黄酮、原花青素和花色苷的含量.结果]浓度60％乙醇粗提物对DPPH·和·OH的清除效果最好,清除率分别为（68.45％±2.68％）和（49.12％±2.26％）;乙酸乙酯萃取物对O2-·的清除率最大为（79.64％±5.16％）.浓度60％乙醇粗提物中花色苷浓度为3.65 mg/ml,而乙酸乙酯萃取物中含有高浓度黄酮,浓度为3.42 mg/ml.结论]龙葵果提取物中起主要抗氧化作用的是花色苷和黄酮.
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| 关 键 词： | 龙葵（Solanum nigrum L ） 抗氧化 花色苷 黄酮 |
Study on Antioxidant Activity of Extracts from Solanum nigrum L.Berries and Analysis of the Active Ingredients |
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| TENG Fei,WANG Ping.Study on Antioxidant Activity of Extracts from Solanum nigrum L.Berries and Analysis of the Active Ingredients[J].Journal of Anhui Agricultural Sciences,2014(19):6217-6219. |
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| Authors: | TENG Fei WANG Ping |
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| Institution: | et al (Forestry School, Northeast Forestry Univer Sity, Harbin, Heilongjiang 150040) |
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| Abstract: | Objective] The antioxidant activity of the different extracts from Solanum nigrum L.berries was compared and the active ingredients were determined.Method] The Solanum nigrum L.berries were extracted by 60% ethanol.Then 60% ethanol extracts were extracted by n-butanol,ethyl acetate and chloroform,respectively.DPPH free radicals,hydroxyl free radicals and superoxide radicals scavenging assays were investigated.In order to determine phenolic acid,flavonoids,proanthocyanidin and anthocyanins,the method of gallic acid,rutin colorimetric,hydrochloric acid-vanillin and pH-differential spectrophotometry were applied in this work.Results] Ethanol extract had the highest DPPH free radicals and hydroxyl free radicals scavenging activity （scavenging activities of 68.45%-± 2.68% and 49.12 ±-2.26%,respectively）.The ethyl acetate extracts had the highest superoxide radicals scavenging activity （79.64% ± 5.16%）.Ethyl acetate extract contained the highest concentration of flavonoids,while 60% ethanol extract contained the highest concentration of anthocyanins.Conclusion] This showed that anthocyanins and flavonoids had a positive effect against oxidative. |
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| Keywords: | Solanum nigrum L Antioxidant activity Anthocyanins Flavonoids |
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