| 蝴蝶兰组培苗快繁高效安全技术 |
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| 引用本文： | 束冰,张金云,谢光坤,杨大庆.蝴蝶兰组培苗快繁高效安全技术[J].安徽农业科学,2014(19):6170-6171,6173. |
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| 作者姓名： | 束冰 张金云 谢光坤 杨大庆 |
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| 作者单位： | 安徽省农业科学院园艺研究所,安徽合肥,230031;安徽省兰君园艺有限公司,安徽合肥,230031;安徽省桐城市农业行政执法大队,安徽桐城,231400 |
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| 基金项目： | 安徽省盆栽花卉工程技术研究中心(201206G01005) |
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| 摘 要： | 目的]建立蝴蝶兰组培苗的快繁工厂化生产体系.方法]以蝴蝶兰的花梗为外植体进行组织培养,并进行继代培养、壮苗培养、生根培养、炼苗、出瓶培养,最后分别包装、标识、运输.结果]试验获得了无菌的蝴蝶兰芽苗,经8代继代培养后,进行壮苗培养和生根培养,而后进行炼苗与出瓶培养,最后按照大、中、小3个规格的苗龄分级出圃,对出圃的组培苗按级分别包装、标识、运输,形成了一套完整的详细的蝴蝶兰苗快繁工厂化生产体系.结论]该方法建立了蝴蝶兰组培苗的快繁工厂化生产体系,为蝴蝶兰的进一步开发利用提供了依据.
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| 关 键 词： | 蝴蝶兰(Phalaenopsis amabilis) 组培 快繁 外植体 |
Rapid Propagation and High Efficient Safety Technology of Phalaenopsis amabilis Tissue Culture Plantlet |
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| Institution: | SHU Bing, ZHANG Jin-yun et al (Horticulture Institute, Anhui Academy of Agricultural Sciences, Hefei, Anhui 230031 ) |
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| Abstract: | Objective] To establish rapid propagation production system of Phalaenopsis amabilis tissue culture plantlet.Method] With stalk of P.amabilisas explant,tissue culture,subculture,strong seedling culture,rooting culture,exercising seedling,flask culture,packaging,identifying and transportation were conducted.Result] Sterile butterfly orchid bud seedling was obtained,after 8 generation propagation,strong seedling culture,rooting culture,exercising seedling,flask culture were conducted.At last,outplanting was carried out according to three grade of large,medium and small.The tissue culture seedlings were packaged,identified and transported,a complete rapid propagation production system for P.amabilis was formed.Conclusion] The rapid propagation production system of P.amabilis tissue culture plantlet was established,which will provide basis for further development and utilization of P.amabilis. |
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| Keywords: | Phalaenopsis amabilis Tissue culture Rapid propagation Explant |
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