| 重组敬钊毒素在毕赤酵母中的表达与纯化研究 |
| |
| 引用本文： | 蒋倩倩,李慧玲,刘莉莉,高宁,程玉鹏.重组敬钊毒素在毕赤酵母中的表达与纯化研究[J].安徽农业科学,2014(19):6166+6169-6166,6169. |
| |
| 作者姓名： | 蒋倩倩 李慧玲 刘莉莉 高宁 程玉鹏 |
| |
| 作者单位： | 黑龙江中医药大学药学院; |
| |
| 基金项目： | 黑龙江中医药大学校基金(201004);黑龙江中医药大学优秀创新人才支持计划(2012RCQ12) |
| |
| 摘 要： | 目的]研究重组敬钊毒素在毕赤酵母中的表达与纯化.方法]通过设计、构建Jztx-Ⅲ/pPIC9k表达载体,经电转化、甲醇诱导后使敬钊毒素在毕赤酵母GS115中进行表达,并利用C-端6×His标签进行分离纯化.结果]Jztx-Ⅲ可以在毕赤酵母GS115中高效表达,经亲和层析纯化后其产量可达到95 mg/L.结论]该试验结果解决了敬钊毒素资源有限产量较低的问题.
|
| 关 键 词： | 蜘蛛毒素 毕赤酵母 基因工程 |
The Expression and Purification of Jingzhao Toxin in Pichia pastoris |
| |
| JIANG Qian-qian,CHENG Yu-peng.The Expression and Purification of Jingzhao Toxin in Pichia pastoris[J].Journal of Anhui Agricultural Sciences,2014(19):6166+6169-6166,6169. |
| |
| Authors: | JIANG Qian-qian CHENG Yu-peng |
| |
| Institution: | et al ( College of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang 150040 ) |
| |
| Abstract: | Objective] To study expression and purification of Jingzhao toxin in Pichia pastoris.Method] Jztx-Ⅲ GS115/pPIC9k plasmid was constructed to express Jingzhao toxin-Ⅲ in Pichia pastoris.A 6 × His tag in C-terminus was employed to purify the recombinant Jztx-Ⅲ.Result] The results showed that Jztx-Ⅲ can be expressed in Pichia pastoris GS115 effectively.The production of Jztx-Ⅲ can reach to 95 mg/L after purified by affinity chromatography.Conclusion] The experiment results solve the problem of lower yield of Jingzhao toxin limited resource. |
| |
| Keywords: | Spider toxin Pichia pastoris Genetic engineering |
| 本文献已被 CNKI 维普 等数据库收录！ |
