| 绶草高频再生体系建立的研究 |
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| 引用本文： | 李慧玲,蒋倩倩,刘莉莉,高宁,程玉鹏.绶草高频再生体系建立的研究[J].安徽农业科学,2014(19):6162+6176-6162,6176. |
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| 作者姓名： | 李慧玲 蒋倩倩 刘莉莉 高宁 程玉鹏 |
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| 作者单位： | 黑龙江中医药大学药学院,黑龙江哈尔滨,150040;黑龙江中医药大学药学院,黑龙江哈尔滨,150040;黑龙江中医药大学药学院,黑龙江哈尔滨,150040;黑龙江中医药大学药学院,黑龙江哈尔滨,150040;黑龙江中医药大学药学院,黑龙江哈尔滨,150040 |
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| 基金项目： | 黑龙江中医药大学校基金(200710);黑龙江中医药大学校基金(201004);黑龙江中医药大学优秀创新人才支持计划(2012RCQ13) |
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| 摘 要： | 目的]建立绶草高频再生体系.方法]以野生绶草的花序轴为外植体,对外植体进行消毒和预培养后,以MS为基本培养基,添加不同浓度的6-BA筛选最适诱导培养基;以MS为基本培养基,添加不同浓度6-BA和IAA筛选最适增殖培养基;以1/2 MS为基本培养基,添加不同浓度NAA筛选最适生根培养基.结果]最适诱导培养基为MS＋ 6-BA2.0,最适增殖培养基为MS＋ 6-BA15＋IAA0.4,最适生根培养基为1/2MS＋ NAA1.5.结论]该研究对绶草优良品种的改良和次级代谢产物研究有重要意义.
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| 关 键 词： | 绶草 再生体系 诱导 |
On Establishing a High Frequency Regeneration System of Spiranthes sinensis |
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| Institution: | LI Hui-ling, CItENG Yu-peng et al ( College of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang 150040 ) |
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| Abstract: | Objective] To establish a high frequency regeneration system of Spiranthes sinensis.Method] With rachis of wild Spiranthes sinensis as explant,disinfection and preculture was conducted.With MS as basic medium,adding different concentration 6-BA,the optimum induction medium was obtained; with MS as basic medium,adding different concentration 6-BA and IAA,the optimum proliferation medium was selected; with 1/2 MS as basic medium,add different concentration NAA for screen out the optimal rooting medium.Result] The optimum medium for induction is MS ＋ 6-BA 2.0 ; the optimum medium for proliferation is MS ＋ 6-BA 1.5 ＋ IAA 0.4 and 1/2 MS ＋ NAA 1.5 is the best rooting medium.Conclusion] The study has significance for improvement and secondary metatolites research of Spiranthes sinensis fine cultivar. |
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| Keywords: | Spiranthes sinensis Regeneration system Induction |
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