| 复方中草药对宝石鲈抗氧化能力及免疫功能的影响 |
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| 引用本文： | 梁拥军,孙向军,孙砚胜,苏建通,史东杰,马峻峰.复方中草药对宝石鲈抗氧化能力及免疫功能的影响[J].安徽农业科学,2009,37(15):7026-7029. |
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| 作者姓名： | 梁拥军 孙向军 孙砚胜 苏建通 史东杰 马峻峰 |
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| 作者单位： | 北京市水产科学研究所,北京,100068 |
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| 基金项目： | 北京市科技计划(研发攻关类) |
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| 摘 要： | 目的]为研究宝石鲈饲料的中草药添加剂提供一定理论依据。方法]试验采用单因素梯度设计，在基础饲料中分别添加质量分数为0．5％、1．0％、2．0％剂量的复方中草药，饲养宝石鲈初始重（175．6±6．6）g]25d后，测定其生长速度，并分析超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、丙二醛（MDA）和溶菌酶（ISZ）活性，考察复方中草药对宝石鲈生长和血清非特异性免疫的影响。结果]添加1．0％复方中草药组宝石鲈的增重率最大，但无显著差异。添加复方中草药可显著提高宝石鲈血清、脾脏、肝脏、肾脏中SOD、溶菌酶和过氧化氢酶活性，其中以1．0％复方中草药组影响最大。说明在饲料中添加该复方中草药有利于宝石鲈抗氧化能力和非特异免疫能力，可提高鱼体对环境的适应能力和应激能力。结论]中草药组方能够增强宝石鲈体液免疫功能，从而增强机体对外来病原菌的防御能力。
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| 关 键 词： | 中草药 宝石鲈 免疫 SOD活性 |
Effect of the Compound Chinese Herbal Medicine on the Antioxidant Capacity and Immune Function of Perch |
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| Institution: | LIANG Yong-jun et al (Aquatic Research Institute of Beijing, Beijing 100068) |
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| Abstract: | Objective] The theoretical basis of the application of the Chinese herbal medicine as feed additive in perch-raising was provided. Method] The design of single-factor gradient experiment,which was 4 treatments including the basic feed(Ⅰ),the doses of the basic feed added with 0.5%(Ⅱ),1.0%(Ⅲ) and 2.0%(Ⅳ) of compound Chinese herbal medicine for perch with the initial body-weight of(175.6±6.6)g for 25 days' raising,was adopted to determine its growth rate and analyze the activity of superoxide dismutase(SOD),catalase(CAT),malondialdehyde(MDA) and lysozyme in its growth(LSZ),and to test the effect of compound Chinese herbal medicine on perch growth and non-specific immunization of serum.Results] The rate of weight-increasing of perch fed with the feed added with 1.0% of compound Chinese herbal medicine was highest among all of treatments,but there was not significant difference in rate of weight-increasing of perch among the treatments.The activity of SOD,lysozyme and catalase in the serum,spleen,liver and kidney of perch fed with the feed added with compound Chinese herbal medicine can be significantly improved and the biggest activity of those was produced by the treatment Ⅲ.It was indicated that the addition of compound Chinese herbal medicine in the feed could improve antioxidant capacity and non-specific immunity of perch,and may strengthen adaptability and the ability in the reaction to environmental stress of the fish.Conclusion] The application of compound Chinese herbal medicine in the feed was able to strengthen the perch's immune function,so as to enhance the body's defenses to pathogens. |
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| Keywords: | Chinese herbal medicine Perch Immunization SOD activity |
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