| 爪哇正青霉Q-5产几丁质酶发酵条件的研究 |
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| 引用本文： | 许天委,张世清,黄俊生.爪哇正青霉Q-5产几丁质酶发酵条件的研究[J].安徽农业科学,2006,34(24):6428-6429,6431. |
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| 作者姓名： | 许天委 张世清 黄俊生 |
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| 作者单位： | 中国热带农业科学院环境与植物保护研究所,热带作物生物技术国家重点实验室,海南,儋州,571737 |
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| 基金项目： | 国家科技部“科技基础性工作和社会公益研究专项”（编号：2004DIB3J073）;科技部“国家科枝基础备件平台工作”（编号：2004DKA30560）资助. |
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| 摘 要： | 通过单因素试验对爪哇正青霉Q-5产几丁质酶的条件进行了研究。结果表明:0.5%(w/v)几丁质粉末对爪哇正青霉Q-5产几丁质酶诱导性最强;pH值在5.0～7.0的范围内酶活力最稳定,pH值为4.0时,酶活力最低;金属离子Mn2+和Cu2+对爪哇正青霉Q-5产几丁质酶有明显的促进作用,而Fe3+和Ca2+则完全抑制几丁质酶的活性;以蛋白胨为氮源时,酶的活力最高可达24 U;吐温-80作为表面活性剂能显著提高几丁质酶活性。
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| 关 键 词： | 单因素 爪哇正青霉 几丁质酶 |
| 文章编号： | 0517-6611（2006）24-6428-02 |
| 收稿时间： | 2006-09-21 |
| 修稿时间： | 2006-09-21 |
Study on the Fermentation Condition of the Chifinase from Eupenicilliumjavanicum Strain Q-5 |
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| XU Tian-wei, et al.Study on the Fermentation Condition of the Chifinase from Eupenicilliumjavanicum Strain Q-5[J].Journal of Anhui Agricultural Sciences,2006,34(24):6428-6429,6431. |
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| Authors: | XU Tian-wei |
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| Institution: | Environmental and Plant Protection Institution, State Key Laboratory of Tropical Crop Biotechnology, CATAS, Danzhou, Hainan 571737 |
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| Abstract: | Single-factor design was used for optimizing cultural condictions of chitinase produced from Eupenicillium javanicum Q-5.The experimental results showed that 0.5 % chitin powder was the best medium for the induction of the chitinase from Eupenicillium javanicum Q-5.The chitinase was stable at the range of pH=5.0～7.0 and the enzyme activity was lower in the pH=4.0.The chitinase activity of Eupenicillium javanicum Q-5 could be enhanced by Mn2+ and Cu2+,but was strongly inhabited by Fe3+ and Ca2+.The tryptone as nitrogen source in the basic medium had the highest chitinase activity(24U) and 0.1 % Tween-80 of surfactants could increase the chitinase activity. |
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| Keywords: | Single-factor Eupenicillium javanicum Chitinase |
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