VirB/D4-dependent protein translocation from Agrobacterium into plant cells |
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| Authors: | Vergunst A C Schrammeijer B den Dulk-Ras A de Vlaam C M Regensburg-Tuïnk T J Hooykaas P J |
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| Institution: | Institute of Molecular Plant Sciences, Leiden University, Clusius Laboratory, Wassenaarseweg 64, 2333 AL, Leiden, Netherlands. |
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| Abstract: | The Agrobacterium VirB/D4 transport system mediates the transfer of a nucleoprotein T complex into plant cells, leading to crown gall disease. In addition, several Virulence proteins must somehow be transported to fulfill a function in planta. Here, we used fusions between Cre recombinase and VirE2 or VirF to directly demonstrate protein translocation into plant cells. Transport of the proteins was monitored by a Cre-mediated in planta recombination event resulting in a selectable phenotype and depended on the VirB/D4 transport system but did not require transferred DNA. |
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