Assessment of milk ring test and some serological tests in the detection of <Emphasis Type="Italic">Brucella melitensis</Emphasis> in Syrian female sheep |
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| Authors: | Ayman Al-Mariri Lila Ramadan Rand Akel |
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| Institution: | (1) Department of Molecular Biology and Biotechnology, Atomic Energy Commission, 6091, Damascus, Syria |
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| Abstract: | Brucella melitensis infection prevalence among Syrian female sheep, to evaluate a number of serological tests and to discuss some epidemiological
aspects of brucellosis, was studied. A total of 2,580 unvaccinated Syrian female sheep sera samples were tested for B. melitensis antibodies detection using four serological methods: the Rose Bengal test (RBT), the serum agglutination test (SAT), the
complement fixation test (CFT) and an indirect enzyme-linked immunosorbent assay (iELISA). In addition, 2,375 milk samples
were collected, then milk ring test (MRT) and bacterial isolation test were employed to evaluate the natural organism shedding.
The samples were considered positive in 66%, 64%, and 60% when we employed the RBT, SAT, and iELISA tests, respectively. Whereas,
the CFT test revealed the smallest number of positive samples. By using the MRT, the total prevalence of brucellosis was nearly
38% of samples. A large variation was observed concerning the studied areas, ranging from 24% in Tartous to 44% in both Damascus
and Damascus rural areas. Brucella was isolated from only 677 samples out of the 2,375 female sheep milk samples. |
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