| 道地药材五鹤续断的染色体制片优化与核型分析 |
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| 引用本文： | 施波,雷盼,石开明,周毅峰,周光来,丁莉.道地药材五鹤续断的染色体制片优化与核型分析[J].湖北农业科学,2010,49(1). |
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| 作者姓名： | 施波 雷盼 石开明 周毅峰 周光来 丁莉 |
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| 作者单位： | 湖北民族学院生物科学与技术学院,湖北,恩施,445000 |
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| 基金项目： | 国家民委项目，生物资源保护与利用湖北省重点实验室(湖北民族学院)资助项目 |
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| 摘 要： | 以恩施地区五鹤续断的根尖为材料,研究了预处理时间和解离时间对其染色体制片的影响,并用此最佳条件进行了五鹤续断根尖染色体核型分析。结果表明,0.1%秋水仙素预处理2.5h、1mol/L盐酸解离30min的处理条件为最佳;五鹤续断染色体数目为18条,核型公式为2n=18=12m+4sm+2st,核型为2A型。
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| 关 键 词： | 五鹤续断 染色体制片 核型 |
Optimization of Chromosomes Sectioning and Karyotpe Analysis of Radix Dipsacus |
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| SHI Bo,LEI Pan,SHI Kai-ming,ZHOU Yi-feng,ZHOU Guang-lai,DIN Li.Optimization of Chromosomes Sectioning and Karyotpe Analysis of Radix Dipsacus[J].Hubei Agricultural Sciences,2010,49(1). |
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| Authors: | SHI Bo LEI Pan SHI Kai-ming ZHOU Yi-feng ZHOU Guang-lai DIN Li |
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| Abstract: | The roots of Wu-He Dipsactts in Enshi were used to study the effect of pre-treating time and the time of dissociation on the slide-preparing of the chromosomes.The results revealed that the pretreatment with 0.1%colchicines 2.5h and 1mol/L HCl at 60 temperature degree 30min preformed well in the sectioning.This technique was employed to determine the karyotype of Wu-He Dipsacus.The results showed that the number of chromosomes in Wu-He Dipsacus was 18 and the formula was 2n=18=12m+4sm+2st,and karyotype asymmetry belonged to 2A type. |
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| Keywords: | Wu-He Dipsacus chromosome sectioning karyotype |
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