| 小麦BNS雄性不育中国春恢复基因的连锁群检测和QTL初步定位 |
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| 引用本文： | 孙慧慧,杨 靖,卫 笑,付庆云,曹银萍,茹振钢,李友勇.小麦BNS雄性不育中国春恢复基因的连锁群检测和QTL初步定位[J].麦类作物学报,2016,36(7):856-865. |
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| 作者姓名： | 孙慧慧 杨 靖 卫 笑 付庆云 曹银萍 茹振钢 李友勇 |
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| 作者单位： | 1. 河南科技学院/河南省高等学校作物分子育种重点学科开放实验室/现代生物育种河南省协同创新中心,河南新乡,453003;2. 河南科技学院/河南省高等学校作物分子育种重点学科开放实验室/现代生物育种河南省协同创新中心,河南新乡453003;四川农业大学农学院,四川成都611130 |
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| 基金项目： | 河南省基础与前沿计划重点项目(122300410011，162300410136) |
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| 摘 要： | BNS是一个新发现的温敏小麦雄性不育系,有良好的不育性和自身转换性,在杂交小麦利用和不育资源研究中有重要价值。为定位BNS的恢复基因,首先以BNS的高恢复系中国春为材料,创建BNS×中国春F2作图群体,建立自交结实率和花粉可育率两个表型BSA池;然后用中国春缺体-四体系检测恢复相关连锁群;最后用这些连锁群上的SSR分子标记筛选BSA池,用检测的连锁标记筛选F2作图群体,进一步定位恢复基因的QTL位点。结果表明,用BNS与中国春缺四体杂交,根据F1不育性检测到4个相关连锁群,分别是1A、1B、2B和7B;利用4个相关连锁群和4个非相关连锁群共8个染色体上的222对SSR分子标记筛选2对共4个BSA池,结果在3个相关连锁群上检测到8对连锁标记;用这8对连锁标记筛选F2群体210个个体植株,检测到5个QTL位点,位于1A、1B和2B染色体上。这些位点中,1个与自交结实率相关,2个与两个表型均相关,是主效QTL位点,另2个与花粉可育率相关,是微效QTL位点。这些结果为BNS恢复基因分子标记选择和精细定位奠定了基础。
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| 关 键 词： | 小麦 BNS雄性不育 恢复基因 SSR分子标记 QTL位点 |
Detection of Linkage Groups and Location of QTLs in Chinese Spring for Restorer Wheat BNS Male Sterility |
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| SUN Huihui,YANG Jing,WEI Xiao,FU Qingyun,CAO Yinping,RU Zhengang,LI Youyong.Detection of Linkage Groups and Location of QTLs in Chinese Spring for Restorer Wheat BNS Male Sterility[J].Journal of Triticeae Crops,2016,36(7):856-865. |
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| Authors: | SUN Huihui YANG Jing WEI Xiao FU Qingyun CAO Yinping RU Zhengang LI Youyong |
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| Abstract: | BNS is a new type of thermo-sensitive wheat male-sterile line,and has excellent sterility and convertibility by itself. It is of important value for the utilization of hybrid wheat and the research of genetic resources. In order to locate the restorer gene of BNS,wheat Chinese Spring(CS),a high restorer line for BNS,was selected as parent material to reform an F2 population by crossing over BNS. In the detection,two bulked segregant analysis(BSA) pools of phenotypes,the self-seedsetting rate and pollen fertility rate were established. First,the linkage groups related to restorer gene(s) were detected by Chinese Spring nulli-tetrasomes. Then,SSR molecular markers on these linkage groups were used to screen two BSA pools,and the linked markers obtained in screening were used to screen F2 population to detect QTL loci of restorer gene(s). The results showed that when CS nulli-tetrasomes was crossed over BNS,there were four recombinations in F1 to be pulled into low self-seedsetting rate,meaning that the four linkage groups on chromosomes 1B,2B,1A and 7B were relative to the recovery of BNS. When 222 pairs of SSR primers from 8 chromosomes were used to screen two phenotypes BSA pools,it was found that there were eight SSR markers on three linkage groups on chromosomes 1B,2B and 1A,which are linked with the restoration of BNS. These eight linkage markers then were used for screening 210 individual plants of F2 population,and five QTL loci were found. Of them,one QTL was relative to self seedsetting rate only,and two major QTLs were relative to two phenotypes,and two minor QTLs were relative to fertile pollen rate only,which were located on chromosomes 1A,1B and 2B,respectively. These results laid a value foundation for MAS(marker-assisted selection) and fine mapping of BNS restorer genes. |
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| Keywords: | Wheat BNS male sterility Restorer gene SSR molecular marker QTL locus |
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