| 一株拮抗香蕉枯萎病菌的细菌HN-1的鉴定和拮抗作用的测定 |
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| 引用本文： | 沈林波,熊国如,董丽莎,孔冉,郭婷,张树珍.一株拮抗香蕉枯萎病菌的细菌HN-1的鉴定和拮抗作用的测定[J].热带农业科学,2012,32(3):44-48,68. |
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| 作者姓名： | 沈林波 熊国如 董丽莎 孔冉 郭婷 张树珍 |
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| 作者单位： | 1. 海南大学农学院,海南海口570228／中国热带农业科学院热带生物技术研究所甘蔗研究中心,海南海口571101
2. 中国热带农业科学院热带生物技术研究所甘蔗研究中心,海南海口,571101 |
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| 基金项目： | 现代农业产业技术体系建设专项资金(No.nycytx-24);中央级公益科研院所基本科研业务费(No.ITBB110509) |
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| 摘 要： | 在香蕉枯萎病园区中,从土壤中分离获得一株细菌HN-1,并进行对峙培养、孢子萌发抑制测定。结果表明:HN-1对香蕉枯萎病菌的菌丝生长、孢子萌发具有良好的抑制效果。经形态特征、生理生化特征和16SrDNA序列测定和分析,将HN-1鉴定为短短芽孢杆菌(Brevibacillus brevis)。与病原真菌对峙培养的结果表明,该菌株对供试的15种植物病原真菌均有很好的抑制作用。
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| 关 键 词： | 香蕉枯萎病 短短芽孢杆菌HN-1 拮抗作用 |
Identification of A Strain HN-1 against Banana Fusarium Wilt Disease and Its Antagonism |
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| SHEN Linbo,XIONG Guoru,DONG Lisha,KONG Ran,GUO Ting,ZHANG Shuzhen.Identification of A Strain HN-1 against Banana Fusarium Wilt Disease and Its Antagonism[J].Chinese Journal of Tropical Agriculture,2012,32(3):44-48,68. |
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| Authors: | SHEN Linbo XIONG Guoru DONG Lisha KONG Ran GUO Ting ZHANG Shuzhen |
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| Institution: | 1 College of Agriculture,Hainan University,Haikou,Hainan 570228; 2 Sugarcane Research Center,Institute of Tropical Biosciences and Biotechnology, CATAS,Haikou,Hainan 571101) |
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| Abstract: | A strain HN-1 was obtained from the soil in a field heavily infected with Fusarium oxysporum f.sp.Cubense(FOC).Antagonism of the strain against FOC was tested via dual-culture,inhibition test on conidia germination.Results showed that HN-1 effectively inhibited mycelium growth and conidia germination.Strain HN-1 was identified as Brevibacillus brevis according to its characteristics in morphology,physiology,biochemistry and 16S rDNA sequence.The strain showed high suppression effect on 15 fungal pathogens in the dual-culture trials with fungal pathogens. |
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| Keywords: | banana wilt disease Brevibacillus brevis HN-1 antagonism |
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