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| 口蹄疫病毒P1+2A基因真核表达载体的构建 | |
| 引用本文： | 龚真莉,刘湘涛,刘磊,尚佑军,尹双辉,田宏,郑海学,陈国栋.口蹄疫病毒P1+2A基因真核表达载体的构建[J].甘肃农业大学学报,2006,41(1):8-11. |
| 作者姓名： | 龚真莉 刘湘涛 刘磊 尚佑军 尹双辉 田宏 郑海学 陈国栋 |
| 作者单位： | 1. 甘肃农业大学动物医学院,甘肃,兰州,730070;中国农业科学院兰州兽医研究所,甘肃,兰州,730046 2. 中国农业科学院兰州兽医研究所,甘肃,兰州,730046 3. 甘肃农业大学动物医学院,甘肃,兰州,730070 |
| 基金项目： | 国家863高技术研究发展计划(2003AA241110) |
| 摘 要： | 通过RT-PCR方法获得了口蹄疫病毒O型,编号为OF3毒的P1+2A区的目的基因,并将其克隆到克隆载体pMD18-T上,再根据表达载体pQE-Trisystem的特性及酶切位点设计合适的表达引物.由表达引物通过PCR技术从克隆载体上扩增出带有特定酶切位点的目的片段,再对载体和目的片段进行酶切处理,处理后由T4 DNA连接酶将二者连接.通过PCR及酶切鉴定,结果证明口蹄疫病毒目的基因片段已被正确克隆到表达载体pQE-Trisystem上. |
| 关 键 词： | 口蹄疫病毒 P1+2A基因 pQE-Trisystem |
| 文章编号： | 1003-4315（2006）01-0008-04 |
| 收稿时间： | 2005-08-24 |
| 修稿时间： | 2005-08-24 |
Construction of eukaryon expression vectors for P1+2A genes of foot-and-mouth disease virus |
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| GONG Zhen-li,LIU Xiang-tao,LIU Lei,SHANG You-jun,YIN Shuang-hui,TIAN Hong,ZHENG Hai-xue,CHEN Guo-dong.Construction of eukaryon expression vectors for P1+2A genes of foot-and-mouth disease virus[J].Journal of Gansu Agricultural University,2006,41(1):8-11. | |
| Authors: | GONG Zhen-li LIU Xiang-tao LIU Lei SHANG You-jun YIN Shuang-hui TIAN Hong ZHENG Hai-xue CHEN Guo-dong |
| Institution: | 1. College of Veterinary Medicine. Gansu Agricultural University,Lanahou 730070; 2. Lanahou Veterinary Research Insistute,Chinese Academy of Agricultural seience,Lanahou 730046,China |
| Abstract: | The antigen DNA fragments of FMDV P1 2A genes were obtained through RT-PCR method.Then the fragments were cloned into the cloning vectors,pMD18-T vectors.Due to the characteristic of the eu-expression vector,pQE-Trisystem,and its enzyme sites,the appropriate primers were designed.Using the expression primers cloned the aimed DNA fragements which contain the right enzyme sites were amplified.The expression vectors and the aimed fragements were digested respectively,and then,they were ligated by the T4 DNA ligatease.Through PCR,restriction analysis and DNA sequencing,the positive cloning plasmid pQE P12A was found. |
| Keywords: | foot-and-mouth disease virus P1 2A gene pQE-Trisystem |
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