| 植物枯萎病拮抗细菌KL-1菌株的分离及鉴定 |
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| 引用本文： | 戴君勇,贲爱玲,吴向华,吴敏敏,陈玲.植物枯萎病拮抗细菌KL-1菌株的分离及鉴定[J].安徽农业科学,2010,38(34):19404-19407. |
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| 作者姓名： | 戴君勇 贲爱玲 吴向华 吴敏敏 陈玲 |
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| 作者单位： | 南京师范大学生命科学学院,江苏南京,210046;南京晓庄学院生物化工与环境工程学院,江苏南京,211171 |
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| 基金项目： | 江苏省高校自然科学研究项目，江苏省高校"青蓝工程"优秀青年骨干教师培养项目资助 |
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| 摘 要： | 目的]筛选、鉴定植物枯萎病菌拮抗细菌,为植物枯萎病生防制剂的研究奠定基础。方法]采用对峙培养法筛选对植物枯萎病菌具有良好抑制作用的生防细菌,通过Biolog细菌自动鉴定系统和16SrDNA序列分析法鉴定其分类地位,并研究该菌株对棉花和小鼠的安全性。结果]从棉花根际土壤中分离出的12株细菌中,有5株对多隔镰刀菌、香石竹尖孢镰刀菌、棉花枯萎病菌具有拮抗活性;其中,KL-1菌株对3株目标病原菌的抑菌活性分别达69.09%、80.78%、78.89%。Biolog细菌自动鉴定系统和16SrDNA序列分析法鉴定结果表明,KL-1菌株为解淀粉芽孢杆菌;生物安全性初步测定结果表明,KL-1菌株对棉花和小鼠安全无毒。结论]解淀粉芽孢杆菌KL-1菌株具有对多种植物枯萎病菌的抑菌活性,且对棉花和小鼠安全无毒,是一株具有研究和开发潜能的生防菌株。
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| 关 键 词： | 植物枯萎病 拮抗细菌 解淀粉芽孢杆菌 鉴定 |
Isolation and Identification of Antifungal Bacterial Strain KL-1 against Plant Wilt Disease |
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| Institution: | DAI Jun-yong et al(Life Science College of Nanjing Normal University,Nanjing,Jiangsu 210046) |
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| Abstract: | Objective]The antifungal bacteria of plant wilt disease was screened and identified to provide foundation for the study on bio-control preparation of plant wilt disease.Method]Duel culture method was adopted to screen the bio-control bacteria with good antifungal effect against plant wilt disease,Biolog microbal identification system and 16SrDNA sequence analysis method were selected to identify its taxonomic status,the biological safety of the strain towards cotton and mice were also determined.Result]12 bacterial strains were isolated from rhizosphere of cotton.Among those strains,5 isolates showed antifungal activities against F.decemcellulare Brick,F.oxysporum f.sp.Diathi,F.oxysporum f.sp.vasinfectum.The antifungal effect of KL-1 strain against three target strains of pathogen reached 69.09%,80.78% and 78.89% respectively.Identification results of Biolog microbal identification system and 16SrDNA sequence analysis method showed that KL-1strain was Bacillus amyloliquefaciens;primary determination results of biological safety also showed that the strain KL-1 was safe and non-toxic towards cotton and mice.Conclusion]KL-1strain of B.amyloliquefaciens had antifungal effect against several pathogens of plant wilt diseases,which was safe and non-toxic towards cotton and mice,being the bio-control strain with research and development potential. |
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| Keywords: | Plant wilt disease Antifungal bacteria Bacillus amyloliquefaciens Identification |
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