Genomic Structure of a Geminivirus in the Genus Begomovirus from Yellow Vein-affected Eupatorium makinor |
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| Authors: | Masatoshi ONUKI Kaoru HANADA |
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| Institution: | (1) Kyushu National Agricultural Experiment Station, 2421 Suya, Nishigoushi, Kikuchi-gun, Kumamoto 861-1192, Japan, JP |
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| Abstract: | A virus from yellow vein-affected Eupatorium makinoi was tentatively designated as Eupatorium-infecting geminivirus (EuGV) on the basis of whitefly transmissibility, electron microscopic observation of geminate particles
in sap and symptomatology. EuGV-speciflc DNA fragments were obtained by polymerase chain reaction (PCR). Based on the restriction
analysis of the PCR products, EuGV was suggested to have a monopartite genome. We determined the putative, complete nucleotide
sequence of the EuGV genome which is comprised of 2766 nucleotides. The EuGV sequence had two virus sense open reading frames
(ORF)(V1, V2), four complementary sense ORFs (C1–C4) and a non-coding region termed the intergenic region. This genome structure
is quite similar to other monopartite begomoviruses already reported. The nucleotide and amino acid sequences were compared
with other begomoviruses. The results supported the conclusion that EuGV is distinct and divergent from other begomoviruses,
whereas potential sequence motifs reported in other geminiviruses are well conserved.
Received 17 March 1999/ Accepted in revised form 12 November 1999 |
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| Keywords: | : monopartite begomovirus sequence comparison conserved motifs Eupatorium makinoi |
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