| 茯砖茶不同萃取物对消化酶活性的影响 |
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| 引用本文： | 傅冬和,刘仲华,黄建安,陈惠衡.茯砖茶不同萃取物对消化酶活性的影响[J].茶叶科学,2008,28(1):62-66. |
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| 作者姓名： | 傅冬和 刘仲华 黄建安 陈惠衡 |
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| 作者单位： | 1. 湖南农业大学教育部茶学重点实验室,湖南,长沙,410128;湖南农业大学园艺园林学院,湖南,长沙,410128
2. 湖南农业大学园艺园林学院,湖南,长沙,410128 |
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| 基金项目： | 科技部重大科技攻关项目
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湖南省教育厅资助项目 |
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| 摘 要： | 将茯砖茶依次用三氯甲烷、乙酸乙酯及正丁醇萃取后制备样品,测定各部分样品对α-淀粉酶及胰脂肪酶活性的影响。研究发现茯砖茶各部分样品对胰脂肪酶活性的影响程度不同,三氯甲烷层稍有抑制作用,活性只有对照的0.82倍,水提物、乙酸乙酯萃取层及正丁醇萃取层有显著的激活作用,正丁醇萃取层部分激活作用最明显,达2.54倍。茯砖茶各部分样品对α-淀粉酶活性均有激活作用,其中以乙酸乙酯萃取层部位的激活作用最强,激活倍数为4.09,其次是水提物,其激活倍数为2.99,水层的激活作用不显著。三氯甲烷萃取层抑制作用不显著,活性只有对照的0.88倍。乙酸乙酯萃取层及正丁醇萃取层部分经过HP-20树脂再分离后,各部分仍有较高的活性。
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| 关 键 词： | 茯砖茶 α-淀粉酶 胰脂肪酶 酶活性 内含成分 |
| 文章编号： | 1000-369X（2008）01-062-05 |
| 收稿时间： | 2007-07-20 |
| 修稿时间： | 2007-09-17 |
The Effect of Different Extracts of Fuzhuan Tea on the Activities of Digesting Enzyme |
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| FU Dong-he,LIU Zhong-hua,HUANG Jian-an,CHEN Hui-heng.The Effect of Different Extracts of Fuzhuan Tea on the Activities of Digesting Enzyme[J].Journal of Tea Science,2008,28(1):62-66. |
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| Authors: | FU Dong-he LIU Zhong-hua HUANG Jian-an CHEN Hui-heng |
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| Abstract: | Fuzhuan Tea was extracted by chloroform,ethyl acetate,1-butanol in turn.The effect of Fuzhuan Tea on the activities of metabolize enzyme was investigated.The results showed that different fractions of Fuzhuan Tea had different effects on pancreatic lipase activity.The sample extracted by chloroform could restrain pancreatic lipase a little and the relative activity was 0.82.The water extracts,the sample extracted by ethyl acetate and 1-butanol showed obvious activation,that of the sample extracted by 1-butanol was the most active.The multiple was 2.54.All fractions of Fuzhuan Tea were active to α-amylase,among them,the sample extracted by ethyl acetate was the most active.The relative activiy was 4.09.The water extracts was the second one and its relative activity was 2.99.That of the layer of water sample was not obvious.The sample extracted by chloroform could restrain α-amylase a little and the relative activity was 0.88.The samples separated from the sample extracted by ethyl acetate and the sample extracted by 1-butanol were active to α-amylase and pancreatic lipase respectively. |
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| Keywords: | Fuzhuan Tea α-amylase pancreatic lipase enzyme activity component |
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