Characterisation ofFusarium oxysporum isolated from carnation in Australia based on pathogenicity,vegetative compatibility and random amplified polymorphic DNA (RAPD) assay |
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| Authors: | G F Kalc Wright D I Guest D L S Wimalajeewa R van Heeswijck |
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| Institution: | (1) School of Botany, University of Melbourne, 3052 Parkville, Australia;(2) Institute of Plant Sciences, Burnley Gardens Swan St., 3121 Burnley, Australia;(3) Department of Horticulture, Viticulture and Oenology, University of Adelaide, Waite Campus, 5064 Glen Osmond, Australia |
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| Abstract: | Isolates ofF. oxysporum collected from symptomless carnation cuttings from Australian carnation growers properties, together with isolates from national collections, were screened for pathogenicity and grouped according to vegetative compatibility and random amplified polymorphic DNA (RAPD) patterns. The collection of 82 Australian isolates sorted into 23 different vegetative compatibility groups (VCGs). Of 69 isolates tested for pathogenicity, 24 were pathogenic to carnations, while the remaining 45 were non-pathogenic. All pathogenic isolates were within two VCGs, one of which was also compatible with an isolate obtained from an international culture collection, and which is known to represent VCG 0021 and race 2. Race status of the two pathogenic VCGs remains unknown. The RAPD assay revealed distinct DNA banding patterns which could distinguish pathogenic from non-pathogenic isolates as well as differentiate between isolates from the two pathogenic VCGs. |
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| Keywords: | Dianthus caryophyllus Fusarium wilt |
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