| 拟南芥春化基因vrn2的克隆及相关分析 |
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| 引用本文： | 杨.拟南芥春化基因vrn2的克隆及相关分析[J].热带作物学报,2003,24(2):46-50. |
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| 作者姓名： | 杨 |
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| 作者单位： | 1. 中国热带农业科学院热带园艺研究所,海南,儋州,571737;中国热带农业科学院热带作物生物技术国家重点实验室,海口,571101
2. 中国热带农业科学院热带作物生物技术国家重点实验室,海口,571101 |
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| 摘 要： | 进行了拟南芥春化基因vrn2(Vernalization2)在拟南芥和唐菖蒲基因组DNA分子上的定位研究。以拟南芥(Arabidopsis thaliana L．)为材料，根据已报道的vrn2基因序列，设计并合成一对特异引物，通过PCR方法扩增出全长3154bp的特异片段。Southern杂交结果表明，在拟南芥基因组中，用HindⅢ酶切消化的DNA在约9．0Kb处有一条杂交带，用BamHI酶切消化的DNA在约2．5Kb处有杂交信号，在唐菖蒲基因组中，用HindⅢ酶切消化的DNA在约2．7Kb处有杂交信号出现。杂交结果说明vrn2在拟南芥基因组中是单拷贝基因，在唐菖蒲基因组中也含有此基因或是具有与vrn2同源的序列。
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| 关 键 词： | 拟南芥 春化基因vrn2 基因克隆 PCR Southern杂交 序列分析 基因定位 成花生理 唐菖蒲 |
| 修稿时间： | 2002年8月28日 |
Cloning and Analysis of Vernalization-Related Gene vrn2 in Arabidopsis thaliana L |
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| Yang Yan , Xu Biyu Jin Zhiqiang.Cloning and Analysis of Vernalization-Related Gene vrn2 in Arabidopsis thaliana L[J].Chinese Journal of Tropical Crops,2003,24(2):46-50. |
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| Authors: | Yang Yan Xu Biyu Jin Zhiqiang |
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| Institution: | Yang Yan 1,2 Xu Biyu 2 Jin Zhiqiang 2 |
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| Abstract: | Genomic total DNA was extracted from Arabidopsis thaliana L.and Gladiolus hybridus.A pair of primers were designed and synthesized based on the reported sequence of vrn2.By polymerase chain reaction,the specific PCR product of vrn2gene was obtained and was about 3100bp.The product was cloned to pGEM-T easy vector,and then sequenced.As the result of sequencing,the PCR product containing the primers sequences was3154bp long.Southern hybridization showed that a single copy of vrn2gene was in the genome of Arabidopsis thaliana and that vrn2gene or its homologous sequence was also in the genome of Gladiolus hybridus. |
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| Keywords: | Arabidopsis thaliana L Gladiolus hybridus cloning sequencing Southern blotting |
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