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紫茎泽兰提取物对棉蚜的毒力及其灭蚜机理研究
引用本文:王一丁,高平,郑勇,刘昆,刘世贵.紫茎泽兰提取物对棉蚜的毒力及其灭蚜机理研究[J].植物保护学报,2002,29(4):337-340.
作者姓名:王一丁  高平  郑勇  刘昆  刘世贵
作者单位:1. 四川师范大学化学与生命科学学院,成都,610068
2. 四川大学生命科学学院,成都,610064
基金项目:国家863计划资助项目(2001AA246017)
摘    要:紫茎泽兰Eupatorium adenophorum提取物对棉蚜Aphis gossypii有很高的毒杀作用。紫茎泽兰乙醇总提物、其氯仿萃取物以及从氯仿萃取物中分离得到的紫茎泽兰素A对棉蚜无翅成蚜的致死中浓度(LC_(50)浸渍法)分别为167.9、224.7、362.8mg/L。试验表明,紫茎泽兰素A对棉蚜钠-钾-三磷酸腺苷酸酶(Na-K-ATPase)活性有明显的抑制作用。棉蚜经2.00mg/ml紫茎泽兰素A溶液处理12h后,其体内的Na-K-ATPase活性仅为对照组棉蚜Na-K-ATPase活性的21.60%。

关 键 词:紫茎泽兰  紫茎泽兰素A  棉蚜  Na-K-ATPase  毒力
收稿时间:2001/5/17 0:00:00
修稿时间:2001年5月17日

The toxicities of the extracts from Eupatorium adenophorum against Aphis gossypii and their aphid-killing mechanism
Wang Yiding,Gao Ping,Zheng Yong,Liu Kun and Liu Shigui.The toxicities of the extracts from Eupatorium adenophorum against Aphis gossypii and their aphid-killing mechanism[J].Acta Phytophylacica Sinica,2002,29(4):337-340.
Authors:Wang Yiding  Gao Ping  Zheng Yong  Liu Kun and Liu Shigui
Institution:College of Life Science, Sichuan University, Chengdu 610064, China,College of Life Science, Sichuan University, Chengdu 610064, China,College of Life Science, Sichuan University, Chengdu 610064, China,College of Life Science, Sichuan University, Chengdu 610064, China and College of Life Science, Sichuan University, Chengdu 610064, China
Abstract:The toxicities of several extracts from Eupatorium adenophorum against cotton aphids ( Aphis gossypii) and the effect of Eupatorin A on Na-K-ATPase were studied. The total ethanol extracts from E. adenophorum, chloroform extract from the total ethanol extract and Eupatorin A from chloroform extract are all highly effective against cotton aphids. The median lethal concentrations a-gainst apterous aphid are 167.9 , 224.7, 362.8mg/L respectively, 48 hours after treatment by dipping method. By means of colorimetry Eupatorin A was found to be able to inhibit the activity of Na-K-ATPase of cotton aphids both in vitro and in vivo.
Keywords:Eupatorium adenophorum  Eupatorin A  Aphis gossypii  toxicity  Na-K-ATPase  
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