| 鸡痘病毒282E4弱毒株TK基因限制性酶切图谱分析 |
| |
| 引用本文： | 孙明,金宁一.鸡痘病毒282E4弱毒株TK基因限制性酶切图谱分析[J].中国兽医学报,1994,14(1):36-40. |
| |
| 作者姓名： | 孙明 金宁一 |
| |
| 作者单位： | 北京军区兽医检验所，农牧大学军事兽医研究所 |
| |
| 摘 要： | 以限制性内切酶ＢａｍＨＩ、Ｘｂａｌ、Ｃｌａｌ、Ｈ１ｎｄⅢ、Ｎｃａｌ对含有鸡痘病毒（ＦＰＶ）２８２Ｅ４弱毒株３．７ｋｂＨｉｎｄⅢＴＫ基因片段的重组质粒ｐＳＬ１进行单酶和它们之间双酶酶切。结果表明：３．７ｋｂＨｉｎｄⅢＴＫ基因片段上有２个Ｃｌａｌ切点，２个Ｘｂａｌ切点，１个Ｎｃａｌ切点，没有ＢａｍＨＩ切点。随后，用澳大利亚ＦＰＶ２．２ｋｂＨｉｎｄⅢ＋ＣｌａｌＴＫ基因作探针，对各种酶切片段进行Ｓｏｕｔｈｅｒｎ印迹杂交，进一步确定ＴＫ基因位于２．２ｋｂＨｉｎｄⅢ＋Ｃｌａｌ片段中。杂交结果与限制性酶切图谱分析结果相一致。该基因的限制性酶切图谱与澳大利亚ＦＰＶ疫苗株的基本相同。
|
| 关 键 词： | 鸡痘病毒，TK基因，限制性酶切图谱，分析 |
Restriction Endonulease Mapping of the Thymidine Ki-.nase Gene of the 282E4 Attenuated
Fowlpox Virus |
| |
| Sun Ming et al.Restriction Endonulease Mapping of the Thymidine Ki-.nase Gene of the 282E4 Attenuated
Fowlpox Virus[J].Chinese Journal of Veterinary Science,1994,14(1):36-40. |
| |
| Authors: | Sun Ming |
| |
| Abstract: | The pSL1,a recombinant plasmid containing 3.7 kb Hind III fragment,
was digested with Hind III, Bam HI,Clal ,Xbal and Ncal respectively and single anddouble
restriction endonuclease mappings of the 3.7 kb Hind III fragment were analysedby agarose gel
electrophoresis and Southern Blotting hybridization using the Digoxi-genin-labelled Australia
TK gene. The results showed that there were two Xbal sites,two Clal sites and one Ncal site. It
was basically same as the counterpart of Australiafowlpox virus vaccine strain. This would lay
a foundation for the construction of afowolpx virus vector. |
| |
| Keywords: | fowlpox virus thymidine kinase gene restriction endonuclease map- ping |
| 本文献已被 CNKI 维普 等数据库收录！ |
