| 外源DNA导入创造抗枯萎病西瓜种质资源 |
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| 引用本文： | 肖光辉,吴德喜,刘建雄,郑素秋,肖兰异.外源DNA导入创造抗枯萎病西瓜种质资源[J].湖南农业大学学报(自然科学版),1999,25(6):453-457. |
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| 作者姓名： | 肖光辉 吴德喜 刘建雄 郑素秋 肖兰异 |
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| 作者单位： | 1. 湖南省园艺研究所,长沙,410125
2. 湖南农业大学植物科学技术学院,长沙,410128 |
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| 基金项目： | 湖南省科委基金!( 0 1 942 0 4 3)，湖南省自然科学基金!( 97JJY2 0 0 6) |
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| 摘 要： | 采用ＤＮＡ浸胚法将供本瓠瓜的总ＤＮＡ导入导入西瓜，Ｄ１代获得一变异株，变异率为０．３２％，Ｄ２代是植株成株期功能叶过氧化物酶同工酶酶带数增加，出现了供体植株的酶带，变异株部分染色体的臂长、臂比、带型与受体相比产生了明显的差异，果实有２２．７％的皮色由深绿变成白色或白皮绿网纹，接近供体瓠瓜的皮色；果实形状有３１．０％发生变异；种子的形状和色泽有３３．３％发生变异。初步认为西瓜的性状变异是供体瓠瓜ＤＮ
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| 关 键 词： | 西瓜 瓠瓜 DNA导入 抗病性 枯萎病 种质资源 |
| 修稿时间： | 1999-05-14 |
Resistance to Wilt Disease Watermelon Germplasm Resources by Exogenous DNA Introduction |
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| Xiao Guanghui,Wu Dexi,Liu Jianxiong,Zheng Shuqiu,Xiao Lanyi.Resistance to Wilt Disease Watermelon Germplasm Resources by Exogenous DNA Introduction[J].Journal of Hunan Agricultural University,1999,25(6):453-457. |
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| Authors: | Xiao Guanghui Wu Dexi Liu Jianxiong Zheng Shuqiu Xiao Lanyi |
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| Abstract: | Through the method of soaking embryo,total DNA of donor bottle gourd was extracted and introduced into watermelon .One variant plant in D 1 offspring with variant ratio of 0.32% was obtained.The band number of peroxidase isozyme in functional leaves of D 2 variant plants increased,and some bands of donor's appeared in variant plants.There were obvious variations in some chromosomes' arm length,arm ratio as well as banding patterns between variant plants and recipient. 22.7% of fruit carpodermis color of D 2 plants changed from dark greento white or white color with green net pattern,which was similar to that of the donor.Variations in the fruit shape was observed in 31.0% of D 2 plants and variations in the seed shape and color were observed in 33.3% of D 2 plants.It suggested that variation in the characters of watermelon be induced by exogenous bottle gourd DNA introduction.The characters of D 4 offspring plants of D 3 1,D 3 2,D 3 3,D 3 4 and D 3 5 variant materials which were selected from D 3 offspring plants cultured in contaminated soil were steady already,and disease resistance showed strong when cultured in contaminated soil.Tested by inoculation of the pathogen to young seedlings,D 3 1 and D 3 2 showed high resistance to wilt disease,while D 3 3 and D 3 4 were middle resistant to the disease. |
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| Keywords: | watermelon bottle gourd DNA introduction disease resistame blight Germplasm enhancement |
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