| 石蜡切片法探究杧果果肉细胞的发育规律 |
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| 引用本文： | 付堯,高兆银,李敏,赵德庆,胡美姣.石蜡切片法探究杧果果肉细胞的发育规律[J].中国果业信息,2019,48(4). |
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| 作者姓名： | 付堯 高兆银 李敏 赵德庆 胡美姣 |
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| 作者单位： | 海南大学热带农林学院;;中国热带农业科学院环境与植物保护研究所、农业部热带作物有害生物综合治理重点实验室;;海南省热带园艺产品采后生理与保鲜重点实验室;海南省热带园艺产品采后生理与保鲜重点实验室,中国热带农业科学院环境与植物保护研究所、农业部热带作物有害生物综合治理重点实验室,中国热带农业科学院环境与植物保护研究所、农业部热带作物有害生物综合治理重点实验室,海南大学热带农林学院,中国热带农业科学院环境与植物保护研究所、农业部热带作物有害生物综合治理重点实验室 |
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| 基金项目： | 国家重点研发计划(2016YFD0400904);海南重大科技计划(ZDKJ2017003);广西科技计划(桂科AB17292039);基本科研业务费专项 (2016hzs1J002、1630042017026); |
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| 摘 要： | 探明杧果果肉细胞分裂期结束时的平均果实大小及细胞分裂期和膨大期的大致时间,以期为杧果发育与生理领域的研究提供细胞学理论依据,并为杧果生产中果实大小的调控及植物生长调节剂的使用提供理论指导。选用“贵妃”杧果为试材,于盛花期后不同天数进行取样;以传统石蜡切片法为基础,在脱水、透明、展片及粘片等具体方法上进行了适合杧果果肉特点的改良,制片后于NI/E智能生物显微镜(日本)下观察杧果果肉细胞的发育情况。试验结果表明：杧果果肉细胞分裂期结束时,平均果实纵径为34.79 ± 0.52 mm～41.71 ± 1.12 mm,平均果实横径为23.03 ± 0.56 mm～24.51 ± 0.59 mm;果肉细胞的分裂期于盛花期后37 d～56 d结束。
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| 关 键 词： | 石蜡切片法 杧果 发育规律 |
| 收稿时间： | 2018/10/10 0:00:00 |
| 修稿时间： | 2018/11/12 0:00:00 |
Exploring the development regular of mango flesh cells by Paraffin sectioning method. |
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| GAO Zhaoyin,LI Min,ZHAO Deqing and HU Meijiao.Exploring the development regular of mango flesh cells by Paraffin sectioning method.[J].China Fruit News,2019,48(4). |
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| Authors: | GAO Zhaoyin LI Min ZHAO Deqing and HU Meijiao |
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| Institution: | Environment and Plant Protection Institute,Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Integrated Pest Management on Tropical Crops,Ministry of Agriculture,Environment and Plant Protection Institute,Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Integrated Pest Management on Tropical Crops,Ministry of Agriculture,Institute of Tropical Agriculture and Forestry,Hainan University,Haikou,Environment and Plant Protection Institute,Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Integrated Pest Management on Tropical Crops,Ministry of Agriculture |
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| Abstract: | Ascertaining the average fruit size at the end of the cell division stage of mango fruit and the approximate time of the cell division and expansion phase. It is expected to provide a cytology theoretical basis for the study of the development and physiological of the mango fruit. It also provides theoretical guidance for the regulation of fruit size and the use of plant growth regulators in mango production. The "Guifei mango was selected as the experimental material, and samples were taken on different days after the flowering period. Based on the traditional paraffin section method, the specific methods such as dehydration, transparency, section flatting and section adhesion are improved for the characteristics of the mango flesh.After the preparation, the development of the mango flesh cells was observed under the NI/E intelligent biological microscope (Japan). The results showed that the average fruit longitudinal diameter was 34.79 ± 0.52 mm~41.71 ± 1.12 mm at the end of the cell division stage, and the average fruit transverse diameter was 23.03 ± 0.56 mm~24.51 ± 0.59 mm. The cell division stage ends at 37 ~ 56 days after the flowering period |
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| Keywords: | paraffin sectioning method mango fruit developmental regular |
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