Separation of turkey heterophils from blood using two-step Ficoll-Hypaque discontinuous gradients |
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| Authors: | K S Latimer I M Kircher C B Andreasen |
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| Institution: | Department of Veterinary Pathology, College of Veterinary Medicine, University of Georgia, Athens 30602. |
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| Abstract: | A method is presented to separate turkey heterophils from anticoagulated whole blood using two-step Ficoll-Hypaque discontinuous gradients and ammonium chloride lysis of contaminating erythrocytes. Heterophils can be isolated from multiple blood samples within 3 to 4 hours. Using this technique, 66.4 +/- 18.4% (mean +/- standard deviation) of blood heterophils were harvested. Final cell isolates averaged 96.0 +/- 2.9% heterophils with few contaminating eosinophils (2.5 +/- 2.3%) or basophils (1.6 +/- 1.8%). Cell viability, as determined by trypan blue dye exclusion, was 98.0 +/- 1.4%. |
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