| 鹅源大肠埃希菌的分离与鉴定 |
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| 引用本文： | 成大荣,朱善元,王芳,钱金梅,牟志霞.鹅源大肠埃希菌的分离与鉴定[J].动物医学进展,2008,29(10). |
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| 作者姓名： | 成大荣 朱善元 王芳 钱金梅 牟志霞 |
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| 作者单位： | 1. 扬州大学兽医学院,江苏,扬州,225009
2. 江苏畜牧兽医职业技术学院,江苏,泰州,225300 |
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| 基金项目： | 扬州大学科技创新培育基金 |
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| 摘 要： | 从临盏床疑似大肠埃希菌感染的病雏鹅的组织分离到31株细菌，通过培养特性和生化试验确定其中27株为大肠埃希菌。血清学试验表明，其中7株（25．93％）为026型，6株（22．22％）为078型，4株（14．81％）为01型，3株（11．11％）为018型，2株（7．41％）为0117型，5株（18．52％）未能定型。应用PCR方法检测了27株大肠埃希菌的F1菌毛与毒力岛HPI基因，结果表明24株（88．89％）为F1^＋大肠埃希菌，7株（25．939／5）为HPI^＋大肠埃希菌（其中4株为F1^＋）。药敏试验表明，所有菌株均对头孢唑林、呋喃妥因敏感，部分菌株对庆大霉素、环丙沙星敏感，而对多黏菌素、四环素、林可霉素均为耐药。
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| 关 键 词： | 鹅 大肠埃希菌 分离 鉴定 |
Isolation and Identification of Escherichia coli from Goose |
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| CHENG Da-rong,ZHU Shan-yuan,WANG Fang,QIAN Jin-mei,MU Zhi-xia.Isolation and Identification of Escherichia coli from Goose[J].Progress In Veterinary Medicine,2008,29(10). |
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| Authors: | CHENG Da-rong ZHU Shan-yuan WANG Fang QIAN Jin-mei MU Zhi-xia |
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| Institution: | 1.College of Veterinary Medicine;Yangzhou University;Yangzhou;Jiangsu;225009;China;2.Jiangsu Animal Husbandry and Veterinary College;Taizhou;225300;China |
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| Abstract: | A total of 31 strains of bacteria were isolated from diseased or deceased geese,and 27 isolates were characterized to be Escherichia coli by Gram staining,culture character and biochemical reaction.The data of O serotype test showed that 7(25.93%) were O26,6(22.22%) were O78,4(18.52%) were O1,3(11.11%) were O18 and 2(7.41%) were O117,while 5(18.52%) were undetermined.In addition,all the isolates were submitted to PCR for F1 and HPI genes,and the data showed that 24 isolates(88.89%) were F1-positive and 7 is... |
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| Keywords: | goose Escherichia coli isolation identification |
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