| “金丰一号”金银花组织培养技术研究 |
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| 引用本文： | 陈明霞,张晓丽,龚玉佳,刘文英,赵喜亭,李明军.“金丰一号”金银花组织培养技术研究[J].北方园艺,2010(23):126-129. |
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| 作者姓名： | 陈明霞 张晓丽 龚玉佳 刘文英 赵喜亭 李明军 |
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| 作者单位： | 河南师范大学,生命科学学院,河南,新乡,453007;河南省高校道地中药材保育及利用工程技术研究中心,河南,新乡,453007;河南师范大学,生命科学学院,河南,新乡,453007 |
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| 基金项目： | 河南省重点科技攻关资助项目，新乡市科技攻关资助项目 |
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| 摘 要： | 对金银花中的优良品种"金丰一号"的离体培养进行了系统的研究。结果表明:将金银花顶芽剥离成二叶一心后,在75%酒精中灭菌30 s,再用0.1%升汞处理15 min,然后无菌水冲洗5~6次,接种于MS+6-BA 1.0 mg/L+NAA 0.2 mg/L培养基上进行培养,无菌苗得率达78.90%;适于试管苗快繁的培养基是MS+6-BA 0.5 mg/L+NAA 0.1 mg/L+生物-素D 2.0mg/L,繁殖周期为28 d,繁殖系数达到7.37;适于生根的培养基为1/2 MS+IBA 3.0 mg/L,生根率达100%。
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| 关 键 词： | 金银花 组织培养 快速繁殖 |
Study on Tissue Culture of Lonicera japonica Thunb.'Jinfeng-1' |
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| CHEN Ming-xia,ZHANG Xiao-li,GONG Yu-jia,LIU Wen-ying,ZHAO Xi-ting,LI Ming-jun.Study on Tissue Culture of Lonicera japonica Thunb.'Jinfeng-1'[J].Northern Horticulture,2010(23):126-129. |
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| Authors: | CHEN Ming-xia ZHANG Xiao-li GONG Yu-jia LIU Wen-ying ZHAO Xi-ting LI Ming-jun |
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| Institution: | CHEN Ming-xia1,2,ZHANG Xiao-li1,GONG Yu-jia1,LIU Wen-ying1,ZHAO Xi-ting1,LI Ming-jun1-2(1.College of Life Science,Henan Normal University,Xinxiang,Henan 453007,2.Engineering Technology Research Center of Nursing and Utilization of Genuine Chinese Crude Drugs,Henan University,Henan 453007) |
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| Abstract: | We studied the in vitro culture systematically on lonicera joponica of this eximious varietal 'Jinfeng-1' for the first time.The results showed that dip the apical buds with two leaves from farmland in the ethanol of 75% for 30 s at first,then in the HgCl2 of 0.1% for 15 minutes,and inoculated on MS+6-BA 1.0 mg/L+NAA 0.2 mg/L after washed in the germfree water for 5~6.According to these steps,we could get plantlets and the yield of vaccine was up to 78.90%;The best medium for rapid propagation of Lonicera j... |
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| Keywords: | Lonicera japonica Thunb tissue culture rapid propagation |
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