Detection of HMW glutenin subunit variations among 205 cultivated emmer accessions (Triticum turgidum ssp. dicoccum) |
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| Authors: | Q Y Li Y M Yan A L Wang X L An Y Z Zhang S L K Hsam and F J Zeller |
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| Institution: | Key Laboratory of Genetics and Biotechnology, College of Life Science, Capital Normal University, Beijing, China;;Division of Plant Breeding and Applied Genetics, Technical University of Munich, D-85350 Freising-Weihenstephan, Germany;;Corresponding authors, E-mail:; |
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| Abstract: | The high molecular weight glutenin subunits (HMW‐GS) encoded by Glu‐1 loci among 205 accessions of cultivated emmer wheat (Triticum turgidum ssp. dicoccum Schrank) collected from different regions of Europe and China were separated and characterized by SDS‐PAGE in combination with two‐dimensional gel electrophoresis (A‐PAGE × SDS‐PAGE) and acidic capillary electrophoresis. High genetic polymorphisms in HMW‐GS compositions were found. A total of 40 alleles (6 for Glu‐A1 and 34 for Glu‐B1) and 62 subunit combinations (genotypes) were detected, some of which were not previously described. At Glu‐A1 locus, two novel alleles, designated Glu‐A1x coding for the subunit 1A × 1.1 and Glu‐A1y coding for the subunit 1A × 2.1′ were found while seven new subunits (1B × 17*, 1B × 6′, 1B × 13′, 1B × 20*, 1By9*, 1By14.1 and 1By8.1) and 20 novel alleles at Glu‐B1 locus were detected. In particular, some additional protein components were detected, which probably were 1Ay subunits encoded by Glu‐A1 locus. The introduction of both Ax and Ay subunits from tetraploid wheats into hexaploid wheats may increase the genetic variability of gluten genes and consequently improve flour technological properties. |
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| Keywords: | Triticum dicoccum acidic capillary electrophoresis high molecular weight glutenin subunits SDS-PAGE two-dimensional electrophoresis |
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