| 2010—2011年猪场中猪源新型甲型流感病毒流行情况调查 |
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| 引用本文： | 张红娜,柴同杰,李欣,王浩,孙玲玉,李欣贤.2010—2011年猪场中猪源新型甲型流感病毒流行情况调查[J].中国畜牧兽医,2013,40(3):189-191. |
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| 作者姓名： | 张红娜 柴同杰 李欣 王浩 孙玲玉 李欣贤 |
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| 作者单位： | 山东农业大学动物科技学院,山东泰安,271000 |
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| 基金项目： | 国家重点实验室环境保护开放基金，环境微生物学和安全控制的应用，重大动物人兽共患病的防控技术研究及应用子课题"甲型HIN1流感等人兽共患病病原气溶胶传播监测的基础与防控研究，国家科技支撑计划子课题 |
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| 摘 要： | 为深入了解猪源新型甲型流感病毒的流行情况,本研究对山东省9个地市40个猪场进行了猪源新型甲型流感病毒的感染情况调查。在养猪场采集有流感症状猪的血液样品(263份)和鼻咽拭子(235份),分别用HI试验来检测血清抗体滴度和荧光定量PCR技术检测鼻咽拭子中的流感病毒的含量。结果表明,血清阳性率为23.07%～63.33%,总阳性率为41.6%;荧光定量PCR检测鼻咽拭子阳性率为10.0%～64.28%,总阳性率为30.63%。这为了解山东省猪源新型甲型流感病毒的发生和分布,并采取相应的公共卫生措施提供了依据。
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| 关 键 词： | 猪源新型甲型流感病毒 血清 鼻咽拭子 荧光定量PCR HI试验 |
| 收稿时间： | 2012-08-07 |
Prevalence Investigation of Novel Swine-origin Influenza A (H1N1) Virus in Some Pig Farms in Shandong Province from 2010 to 2011 |
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| ZHANG Hong-na
,
CHAI Tong-jie
,
LI Xin
,
WANG Hao
,
SUN Ling-yu
,
LI Xin-xian.Prevalence Investigation of Novel Swine-origin Influenza A (H1N1) Virus in Some Pig Farms in Shandong Province from 2010 to 2011[J].China Animal Husbandry & Veterinary Medicine,2013,40(3):189-191. |
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| Authors: | ZHANG Hong-na CHAI Tong-jie LI Xin WANG Hao SUN Ling-yu LI Xin-xian |
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| Institution: | College of Animal sciences, Shandong Agricultural University, Tai'an 271000, China |
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| Abstract: | To better understand, we investigated the SO (H1N1) IV in 40 pig farms from 9 cities in Shandong province. Serum samples (263) and nasal swabs(235)from pigs with flu-like symptoms were collected, respectively. The positive rates of nasal swabs and blood samples were measured using Real-time RT-PCR and HI-test, respectively. Results demonstrated that, the positive rates of serum samples were 23.07% to 63.33%, accounting for 30.63%; the positive rates of nasal swabs were 10.0% to 64.28%, accounting for 30.63%. This result provided the basis to understand the SO (H1N1) IV distribution in Shandong province. |
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| Keywords: | novel swine-origin influenza A (H1N1) virus serum nasal swab Real time RT-PCR HI-test |
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