| 基于ITS序列分析探讨我国栽培凤尾菇的分类地位 |
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| 引用本文： | 黄龙花,吴清平,杨小兵,张菊梅.基于ITS序列分析探讨我国栽培凤尾菇的分类地位[J].食用菌学报,2009,16(2). |
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| 作者姓名： | 黄龙花 吴清平 杨小兵 张菊梅 |
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| 作者单位： | 1. 中国科学院南海海洋研究所,广东广州,510301;广东省微生物研究所,广东省菌种保藏与应用重点实验室,广东广州,510070;中国科学院研究生院,北京,100049
2. 广东省微生物研究所,广东省菌种保藏与应用重点实验室,广东广州,510070 |
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| 摘 要： | 针对我国栽培凤尾菇(Pleurotus sajor-caju)及其近缘种共10个菌株的ITS序列,应用特异引物进行PCR扩增,对其产物进行测序,并从GenBank上下载侧耳属(Pleurotus)3个种7条ITS序列、韧伞属(Lentinus)的4条ITS序列和香菇属(Lentinula)的3条ITS序列,以冬虫夏草(Cordyceps sinensis)和蛹虫草(Cordyceps militaris)为外类群,用NJ(Neighbor-joining)法构建系统发育树,结果表明:凤尾菇在侧耳属分支上与侧耳属内种的相似性达98.5%以上,韧伞属和香菇属的属内相似性也均为98%以上,而侧耳属和韧伞属、香菇属的属间相似性仅约80%,故凤尾菇应归于侧耳属;在进一步研究中,将该凤尾菇与其近缘种的ITS1-5.8S-ITS2序列作碱基完全比对,结果发现它与肺形侧耳(Pleurotus pulmonarius)的碱基完全一致,而与其它近缘种均存在较大碱基差异,因此,目前我国广泛栽培的凤尾菇与肺形侧耳可能是同物异名种,建议订正其名称,将其拉丁名统一为P. pulmonarius.本研究首次将ITS序列分析和碱基比对结合对凤尾菇的分类地位及拉丁名进行研究,结果表明ITS序列分析在近缘种的分类鉴定上很有价值.
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| 关 键 词： | 凤尾菇 肺形侧耳 ITS序列 系统发育树 |
Taxonomic Status of Pleurotus sajor-caju Cultivated in China Based on ITS Sequence Analysis |
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| HUANG Longhua,WU Qingping,YANG Xiaobing,ZHANG Jumei.Taxonomic Status of Pleurotus sajor-caju Cultivated in China Based on ITS Sequence Analysis[J].Acta Edulis Fungi,2009,16(2). |
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| Authors: | HUANG Longhua WU Qingping YANG Xiaobing ZHANG Jumei |
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| Abstract: | In order to clarify the taxonomic status of Pleurotus sajor-caju, we determined ITS sequences from six Pleurotus (including one strain of P. sajor-caju) and four Lentinula strains cultivated in China. Data were combined with ITS sequences from 11 related strains, including Pleurotus, Lentinus, Lentinula, Cordyceps and Ophiocordyceps, obtained from GenBank to construct a phylogenetic tree using the Neighbor-joining method with Cordyceps militaris and Ophiocordyceps sinensis as the outgroups. The P. sajor-caju test strain was located on a branch that incorporated all the other Pleurotus strains examined with a shared similarity value >98.5%. Similarity values among Lentinus and Lentinula strains was also >98%, but only 80% when all three genera were compared. In a further "complete alignment"study, comparison of sections of ITS1-5.8S-ITS2 sequences from four P. sajor-caju strains with corresponding sequences from strains of P. pulmonarius, P.ostreatus and P. eryngii revealed that the sequences from P. sajor-caju and P. pulmonarius strains were identical but were different from the other Pleurotus strains. Our data indicate that the P. sajor-caju test strain was synonymous with P. pulmonarius and recommend that it be renamed accordingly.- |
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| Keywords: | Pleurotus sajor-caju Pleurotus pulmonarius ITS sequence phylogenetic tree |
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