| 碱性蛋白酶水解猪血细胞最优反应条件的研究 |
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| 引用本文： | 孟祥晨,杨同舟.碱性蛋白酶水解猪血细胞最优反应条件的研究[J].东北农业大学学报,1998,29(2):173-178. |
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| 作者姓名： | 孟祥晨 杨同舟 |
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| 作者单位： | 东北农业大学 |
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| 摘 要： | 研究旨在确立碱性蛋白酶水解猪血细胞的最优反应条件。采用二次旋转正交回归设计，建立了ｐＨ值、温度（Ｔ）、酶—底物浓度比（〔Ｅ〕／〔Ｓ〕）三个因素对水解蛋白收率影响的回归模型。通过分析，确定最优水解条件为：ｐＨ＝８２１，〔Ｅ〕／〔Ｓ〕＝４％，最后，在此最优水解条件下，确定出最佳水解时间ｔ＝９０ｍｉｎ。
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| 关 键 词： | 碱性蛋白酶，水解，猪血细胞 |
THE STUDY ON THE OPTIMUM REACTIONCONDITIONS OF THE HYDROLYSIS OFPORCINE BLOOD CELLS BYALKALINE PROTEASE |
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| Abstract: | おhe aim is determing the optimum reaction conditions in hydrolyzing porcine blood cells by alkaline protease in this thesis The quadratic rotary combination design was used The mathematical model was established for the effect of pH, temperature and enzyme-substrate concentration ration on the yield of Hydrolyzed proteins The optimum hydrolytic conditions were as follows: pH=821, temperature=65 ℃, enzyme-substrate concentration ration=4% On this basis, the effect of different times of hydrolysis on the yield of hyoholyzed proteins was studied, and optimum hydrolytic time was 90 minutes |
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| Keywords: | alkaline protease hydrolysis porcine blood cells |
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