Development of a novel LAMP diagnostic method for visible detection of swine <Emphasis Type="Italic">Pasteurella multocida</Emphasis> |
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| Authors: | Dongbo Sun Jianfa Wang Rui Wu Chunren Wang Xianjing He Jiasan Zheng Huanmin Yang |
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| Institution: | (1) College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing High-tech Industrial Development Zone, Daqing, 163319, People’s Republic of China |
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| Abstract: | A set of four specific primers for six regions of kmt1 gene from a species specific region was designed for developing the
loop-mediated isothermal amplification diagnostic method of swine Pasteurella multocida (Pm-LAMP). After the Pm-LAMP was carried out at 63°C for 1 h, the LAMP products could be visually confirmed using fluorescent
dyes as detection reagent under UV-illumination. In sensitivity, the detection limit of the Pm-LAMP was 10 cfu/mL, and was
1 log less than that of the PCR method. In specificity, the Pm-LAMP did not amplify genomic DNA of swine common respiratory
pathogens. Furthermore, based on results for clinical swab samples (n = 31) using PCR detection as golden standard, relative sensitivity of the Pm-LAMP was 100%, relative specificity of the Pm-LAMP
was 90.9%, and percentage of observation agreement was 93.5% (Kappa = 0.85). The Pm-LAMP method should be a useful diagnostic
tool for rapid and visible detection of swine Pasteurella multocida. |
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