| 猪瘟病毒E2基因DNA疫苗的试验研究 |
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| 引用本文： | 王三虎,李祥瑞.猪瘟病毒E2基因DNA疫苗的试验研究[J].河南农业大学学报,2007,41(5):541-544. |
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| 作者姓名： | 王三虎 李祥瑞 |
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| 作者单位： | 1. 南京农业大学动物医学院,江苏,南京,210095;河南科技学院,河南,新乡,453003
2. 南京农业大学动物医学院,江苏,南京,210095 |
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| 摘 要： | 用构建的E2-pcDNA4.0 DNA疫苗对Balb/c小鼠、家兔和仔猪进行免疫,经3次肌肉接种,间隔15 d免疫后,测定抗体水平.结果表明,构建的DNA疫苗能诱导小鼠产生中和性抗体,免疫家兔最少可抵抗10个最小感染剂量(M ID)的猪瘟兔化弱毒苗的攻击.攻毒试验结果表明,E2-pcDNA4.0 DNA疫苗可抵抗致死剂量的CSFV石门株强毒的攻击.
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| 关 键 词： | 猪瘟 E2基因 DNA疫苗 |
| 文章编号： | 1000-2340（2007）05-0541-04 |
| 收稿时间： | 2007-04-10 |
| 修稿时间： | 2007年4月10日 |
Experimental Study on CSFV E2 DNA Vaccine |
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| WANG San-hu,LI Xiang-rui.Experimental Study on CSFV E2 DNA Vaccine[J].Journal of Henan Agricultural University,2007,41(5):541-544. |
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| Authors: | WANG San-hu LI Xiang-rui |
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| Institution: | 1. College of Verternary Science, Nanjing Agricultural University, Nanjing 210095, China; 2. Henan Institute of Science and Technology, Xinxiang 453003, China |
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| Abstract: | Balb/c mice,rabbits and young pigs were vaccinated by intramuscular injection with the recombinant plasmid E2-pcDNA4.0.After 3 immunizations and detecting the antibody level,the result indicated that the DNA vaccine could induce the mice producing neutralization antibody;and the rabbits resisting at least 10 minimum infectious dose(MID) of the attacking.And the result of the conteracting toxic substances experiments of the young pigs manifested that the E2-pcDNA4.0 DNA vaccine could counteract the attacking of the shimen strain. |
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| Keywords: | CSF E2 gene DNA vaccine |
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