| 与大白菜干烧边性状相关的SSR和SRAP标记分析 |
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| 引用本文： | 李坤,史庆馨,张耀伟.与大白菜干烧边性状相关的SSR和SRAP标记分析[J].中国瓜菜,2010,23(4):6-11. |
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| 作者姓名： | 李坤 史庆馨 张耀伟 |
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| 作者单位： | 1. 东北农业大学园艺学院,哈尔滨,150030
2. 黑龙江省农业科学院园艺分院,哈尔滨,150069 |
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| 基金项目： | "十一五"国家科技支撑计划，黑龙江省高校重点实验室"寒地蔬菜生物学"开放基金项目 |
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| 摘 要： | 以大白菜感干烧边品种A67和抗干烧边品种A53配制的141个F2代单株组成的群体为材料,双亲DNA构建大白菜感病池和抗病池,对104对SSR引物和320对SRAP引物进行筛选,并对与干烧边性状相关的QTL和对应的连锁关系进行了初步分析,获得2个与大白菜干烧边性状相关的QTL,同时获得3个与这2个QTL连锁的标记,其中A29-890和E6-400标记与其最近QTL位点的连锁距离分别为1.5cM和7.1cM。为大白菜抗干烧边性状的分子辅助育种奠定了基础。
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| 关 键 词： | 大白菜 干烧边 SSR SRAP QTL |
SSR and SRAP Markers Linked to Tip-burn Trait in Chinese Cabbage |
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| LI Kun,SHI Qing-xin,ZHANG Yao-wei.SSR and SRAP Markers Linked to Tip-burn Trait in Chinese Cabbage[J].China Cucurbits And Vegetables,2010,23(4):6-11. |
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| Authors: | LI Kun SHI Qing-xin ZHANG Yao-wei |
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| Abstract: | A F2 population including 141 individuals was derived from a cross between susceptible line A67 and resistance line A53,and used for this study.104 pairs SSR primers and 320 pairs SRAP primers were employed in constructing a molecular genetic map and mapping QTL for tip-burn resistance in Chinese cabbage.Twenty five primer pair combinations amplified stable polymorphic bands between two bulks and 2 QTLs concerning tip-burn resistance were detected.Three markers were linked to the QTL’s and two(S29-890 and E6-400)were nearest to QTLs at a distance of 7.1 cM and 1.5 cM.These markers provided a tool for marker-assisted selection of tip-burn resistance in Chinese cabbage breeding. |
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| Keywords: | SSR SRAP QTL |
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