| 当归组织培养技术研究 |
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| 引用本文： | 左利娟,石进朝.当归组织培养技术研究[J].安徽农业科学,2012,40(1):171-173. |
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| 作者姓名： | 左利娟 石进朝 |
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| 作者单位： | 北京农业职业学院,北京,102442;北京农业职业学院,北京,102442 |
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| 基金项目： | 北京农业职业学院基金项目"当归快速繁殖与栽培技术研究" |
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| 摘 要： | 目的]筛选当归组织培养的最佳试验条件。方法]以当归的幼嫩茎段为材料,研究不同激素及浓度对当归增殖和生根的影响。结果]0.1%HgCl2对当归外植体的最佳消毒时间为5 min;MS+6-BA 0.30 mg/L+NAA 0.01 mg/L为诱导与增殖丛生芽的理想培养基;1/2MS+IBA 1.50 mg/L+NAA 0.01 mg/L为丛生芽的最佳生根培养基;移栽驯化中最适宜的栽培基质为腐殖土∶珍珠岩=2∶1。结论]该研究可为当归的工厂化育苗提供理论依据。
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| 关 键 词： | 组织培养 快速繁殖 当归 工厂化育苗 |
Study on Tissue Culture Technology of Angelica sinensis( Oliy.)Diels. |
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| Institution: | ZUO Li-juan et al(Beijing Vocational College of Agriculture,Beijing 102442) |
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| Abstract: | Objective] The paper aimed to select the best experiment condition of tissue culture technology of Angelica sinensis(Oliv.)Diels..Method] With tender stem of A.sinensis(Oliv.)Diels.as material,effects of different kinds and concentrations of hormones on the multiplication and rootage were studied.Result] The best sterilized time for the explants was 5 minutes in 0.1%HgCl2.The optimum medium for induction and multiplication of multiple shoot was MS+6-BA 0.30 mg/L+NAA 0.01 mg/L.The best medium for rooting was 1/2MS+IBA 1.50 mg/L+ NAA 0.01 mg/L.The culture substrate suitable for transplantation was humus soil∶ perlite=2∶ 1.Conclusion] The research could provide a theoretical basis for industrial seedling of A.sinensis(Oliv.)Diels.. |
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| Keywords: | Tissue culture Rapid propagation Angelica sinensis(Oliv )Diels Industrial seedling |
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