| 高效氯氰菊酯降解菌BCC01的分离鉴定研究 |
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| 引用本文： | 刘晶晶,胡美英,李亚楠,陈少华,林惠花,易欣,钟国华.高效氯氰菊酯降解菌BCC01的分离鉴定研究[J].安徽农业科学,2012,40(1):270-272. |
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| 作者姓名： | 刘晶晶 胡美英 李亚楠 陈少华 林惠花 易欣 钟国华 |
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| 作者单位： | 华南农业大学昆虫毒理研究室,广东广州,510642 |
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| 基金项目： | 广东省自然科学基金，国家自然科学基金 |
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| 摘 要： | 目的]分离鉴定高效氯氰菊酯降解菌。方法]从拟除虫菊酯农药污水淤泥中,分离得到15株芽孢杆菌,通过降解HPLC测定降解活性,其中1株对高效氯氰菊酯具有较高活性,对其进行形态、生理生化16S rDNA聚类分析鉴定其种属。结果]该菌株命名为BCC01,鉴定为蜡样芽孢杆菌,能够以高效氯氰菊酯为唯一碳源生长,4 d内对50 mg/L的高效氯氰菊酯降解率为86.8%。结论]BCC01是对高效氯氰菊酯具有高降解活性的蜡样芽孢杆菌。
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| 关 键 词： | 高效氯氰菊酯 生物降解 分离鉴定 |
Isolation and Identification of a Beta-cypermethrin Degrading Strain BCC01 |
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| Institution: | LIU Jing-jing et al(Laboratory of Insect Toxicology,South China Agricultural University,Guangzhou,Guangdong 510642) |
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| Abstract: | Objective] To isolate and identify a beta-cypermethrin degrading strain.Method] 15 beta-cypermethrin(beta-CP) degrading strains were isolated from cypermethrin sewage sludge.One of them could degrade beta-CP efficiently in HPLC detection and was identified based on morphology,physiology and phylogenetic analysis of 16S rDNA gene sequence.Result] The strain named BCC01 was identified as Bacillus cereus.In pure culture with beta-CP as sole carbon source,86.8% of beta-CP at initial concentration of 50 mg/L was degraded in 4 days by BCC01.Conclusion] BCC01 strain belonged to Bacillus cereus with high degradation activity for beta-CP. |
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| Keywords: | Beta-cypermethrin: Biodegrading Isolation and identification |
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