| 柑橘钙调蛋白cDNA的克隆及序列分析 |
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| 引用本文： | 岳海林,孟海军,邓秀新,彭抒昂.柑橘钙调蛋白cDNA的克隆及序列分析[J].园艺学报,2006,33(5):1075-1078. |
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| 作者姓名： | 岳海林 孟海军 邓秀新 彭抒昂 |
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| 作者单位： | ( 华中农业大学作物遗传改良国家重点实验室, 湖北武汉430070; 华中农业大学园艺林学学院, 湖北武汉430070) |
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| 基金项目： | 国家自然科学基金资助项目(30270924,30471202) |
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| 摘 要： | 以温州蜜柑(Citrus unshiu Marc. ) 幼果cDNA第1链为模板, 参照大麦钙调蛋白基因序列(Accession No. M27303) 合成5p端和3p端引物, 以PCR的方法扩增得到柑橘钙调蛋白cDNA, 将其克隆到PMD182T载体上并进行测序。序列分析表明, 柑橘钙调蛋白cDNA全长453 bp, 共编码148个氨基酸, 与大麦和大豆钙调蛋白基因的同源性均高达83%以上, 所编码氨基酸序列同源性达97%以上。以该cDNA作探针进行点杂交, 得到良好的杂交信号。
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| 关 键 词： | 柑橘 钙调蛋白 cDNA克隆 序列分析 |
| 文章编号： | 0513-353X（2006）05-1075-04 |
| 收稿时间： | 2005-12-20 |
| 修稿时间： | 2005-12-202006-03-02 |
Cloning and Sequence Analysis of Citrus Calmodulin cDNA |
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| Yue Hailin,Meng Haijun,Deng Xiuxin,Peng Shu'ang.Cloning and Sequence Analysis of Citrus Calmodulin cDNA[J].Acta Horticulturae Sinica,2006,33(5):1075-1078. |
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| Authors: | Yue Hailin Meng Haijun Deng Xiuxin Peng Shu'ang |
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| Institution: | (National Key Laboratory of Crop Genetic Improvement, Huazhong Agriculture University, Wuhan, Hubei 430070, China;College of Forestry and Horticulture, Huazhong Agriculture University, Wuhan, Hubei 430070, China) |
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| Abstract: | Using the first strand cDNA from the immature fruits of Citrus unshiu Marc. as template, we amplified and cloned the cDNA of Citrus calmodulin gene in this study. The PCR primers were designed according to the sequences of barley calmodulin gene obtained from GenBank (Accession No. M27303). The PCR product was cloned into PMD18-T vector and then sequenced. Sequence analysis indicated that the cDNA of Citrus calmodulin gene contains 453 nucleotides coding for 148 amino acid residues. The nucleotide sequences and their deduced amino acid sequences of the Citrus calmodulin cDNA show an identity of above 83% and 97%, respectively, to those of barley and soybean. Southern dot blot analysis using the cloned cDNA as probe confirmed the results of sequencing analysis. |
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| Keywords: | Citrus Calmodulin cDNA cloning Sequence analysis |
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