| 宁夏枸杞主要栽培品种的DNA多态性分析 |
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| 引用本文： | 尚洁,思彬彬.宁夏枸杞主要栽培品种的DNA多态性分析[J].安徽农业科学,2010,38(6):2801-2802,2805. |
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| 作者姓名： | 尚洁 思彬彬 |
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| 作者单位： | 北方民族大学生物科学与工程学院,宁夏银川,750021;北方民族大学生物科学与工程学院,宁夏银川,750021 |
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| 基金项目： | 北方民族大学资助项目(2006Y040) |
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| 摘 要： | 目的]分析宁夏枸杞（Lycium barbarum L.）主栽品种的DNA多态性。方法]采用RAPD技术,利用11个经筛选在样品间具多态性的10碱基随机引物对4个宁夏枸杞主要栽培品种DNA进行PCR扩增;以Ntsyspc2.1软件计算样品间的遗传相似系数,并进行UP-GMA聚类分析。结果]在样品间共产生99条RAPD标记带,其中多态性带44条,多态性百分比为44.44%;宁夏枸杞栽培品种间遗传关系较近,特别是宁杞1号和宁杞2号,相似性系数达80%。结论]为宁夏枸杞宁夏枸杞种质的利用、保存及种质资源库的构建提供理论基础。
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| 关 键 词： | 宁夏枸杞 随机扩增多态性DNA 遗传多样性 |
Genetic Diversity Analysis of 4 Domestic of Lycium barbarum L. |
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| SHANG Jie et al.Genetic Diversity Analysis of 4 Domestic of Lycium barbarum L.[J].Journal of Anhui Agricultural Sciences,2010,38(6):2801-2802,2805. |
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| Authors: | SHANG Jie |
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| Institution: | College of Life Science and Engineering;Northern University for Nationalities;Yinchuan;Ningxia 750021 |
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| Abstract: | Objective] The research aimed to study the genetic diversity analysis of 4 domestic of Lycium barbarum L..Method] By screening 11 samples being polymorphic with 10 base random primers,the genomic DNAs of 4 domestic of Lycium barbarum L.were amplified.Then the genetic similarity coefficient was calculated by Ntsyspc 2.1 and UPGMA cluster analysis was made.Result] A total of 99 RAPD bands was detected,44 among which were polymorphic,and the percent of polymorphic bands was 44.44%.The genetic relationship a... |
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| Keywords: | Lycium barbarum L Random amplified polymorphic DNA Genetic diversity |
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