| 不同抗性品种柱花草接种炭疽菌后CAT、SOD活性及H2O2、胼胝质含量的变化 |
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| 引用本文： | 张伟丽,郭振飞.不同抗性品种柱花草接种炭疽菌后CAT、SOD活性及H2O2、胼胝质含量的变化[J].扬州大学学报(农业与生命科学版),2007,28(2):73-77. |
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| 作者姓名： | 张伟丽 郭振飞 |
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| 作者单位： | 1. 华南农业大学,生命科学学院,广东,广州,510642;仲恺农业技术学院,生命科学学院,广东,广州,510225
2. 华南农业大学,生命科学学院,广东,广州,510642 |
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| 摘 要： | 以不同抗性品种柱花草为材料,研究接种胶胞炭疽菌后过氧化氢酶(CAT)、超氧化物歧化酶(SOD)活性及H_2O_2、胼胝质含量的变化。结果表明:与对照相比,除高感品种的CAT活性于接种后降低外,高抗品种及中抗品种在试验中均有升高过程,其最大增幅与品种的抗病性呈正相关。除高抗品种的SOD活性于接种后降低外,其他品种在试验中均有升高过程,高感品种的增幅均大于中抗品种,且出现增幅的时间早于中抗品种,SOD活性的增幅与品种的抗病性呈负相关。接种后24 h,高抗与中抗品种接种处理的H_2O_2含量均升至最大值,而高感品种H_2O_2含量达最大值的时间稍滞后,高抗品种能更快、有效地调动H_2O_2介导的防御机制。高抗品种胼胝质含量大约是其他品种的3～5倍,且与抗病性有关,中抗品种TPRC90087接种处理与对照的胼胝质含量差异较小,中抗品种CIAT184和高感品种在受病原菌侵染后可能通过胼胝质的积累来抵御病害。
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| 关 键 词： | 柱花草 胶胞炭疽菌 防御酶 过氧化氢 胼胝质 |
| 文章编号： | 1671-4652(2007)02-0073-05 |
| 修稿时间： | 2007年3月12日 |
Changes in the activities of CAT,SOD and the contents of H2O2,callose in cultivars of Stylosanthes spp differing in the disease resistance after inoculation with Colletotrichum gloeosporiodes |
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| ZHANG Wei-li,GUO Zhen-fei.Changes in the activities of CAT,SOD and the contents of H2O2,callose in cultivars of Stylosanthes spp differing in the disease resistance after inoculation with Colletotrichum gloeosporiodes[J].Journal of Yangzhou University:Agricultural and Life Science Edition,2007,28(2):73-77. |
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| Authors: | ZHANG Wei-li GUO Zhen-fei |
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| Abstract: | The activities of catalase(CAT),superoxide dismutase(SOD),the contents of hydrogen peroxide and callose were measured in four cultivars of Stylosanthes differing in disease resistance,Tardio,CIAT184,TPRC90087 and Graham, following inoculation with conidial suspension of Colletotrichum gloeos poriodes.CAT activity was induced and increased in the high or mediately resistant cultivars during at experimental period except the susceptible cultivar after the inoculation.It seemed that CAT activity was positive correlated with the cultivar resistance to the disease.SOD activity was induced and increased in the mediate or susceptible cultivars at some experimental period except the high resistant cultivars,SOD activity in the susceptible cultivar was increased higher than that in the mediate cultivars,and the maximum of enzymes appeared earlier than mediate in the resistant cultivars,which seemed that SOD activity was positively correlated with the cultivar re- sistance.The contents of hydrogen peroxide in the high and mediate cultivars reached peak at 24 h when inoculated by the pathogen,and susceptible cultivars reached peak at later time than in other three cultivars.It seemed that defense response in high resistant cultivars mediated by hydrogen peroxide wes induced rapidly and effectively.The contents of callose in high resistant cultivars were nearly three to five times of other three cultivars,which could he related to high cultivar resis- tance.But,there was little change in the callose contents when the mediately resistant cultivar TPRC90087 was inoculated by the pathogen.CIAT184 and Graham could defense pathogen infection by accuamateing callose after inoculated by the pathogen. |
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| Keywords: | Stylosanthes spp Colletotrichum gloeosporiodes defense enzymes hydrogen peroxide callose |
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