| 油菜内生生防菌BY-2在油菜体内的定殖与对油菜菌核病的防治作用 |
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| 引用本文： | 江木兰,赵瑞,胡小加,张银波,王国平.油菜内生生防菌BY-2在油菜体内的定殖与对油菜菌核病的防治作用[J].植物病理学报,2007,37(2):192-196. |
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| 作者姓名： | 江木兰 赵瑞 胡小加 张银波 王国平 |
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| 作者单位： | 1 华中农业大学植物科学技术学院, 武昌 430062;2 中国农业科学院油料作物研究所, 武昌 430062;3 国家油料作物遗传改良重点实验室, 武昌 430062 |
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| 基金项目： | 国家科技攻关计划;湖北省科技攻关项目 |
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| 摘 要： | 从油菜植株体内分离出的内生细菌BY-2,经过生物学鉴定为枯草芽孢杆菌(Bacillus subtilis)。用BY-2回接油菜,重新分离到具有BY-2相同形态特征和抑制病原真菌能力的内生菌株。油菜接种后的第10 d,体内的BY-2菌数达(2.24~9.02)×103 cfu/g鲜植株,25 d仍然保持在(3.13~8.59)×103 cfu/g鲜植株。BY-2与油菜核盘菌Sclerotinia sclerotiorum(Lib.) de Bary]对峙培养可以形成直径为3.1 cm的抑菌圈;可使油菜核盘菌菌丝细胞浓缩变短,细胞壁破裂,原生质外溢,从而抑制真菌生长发育;同时还能抑制菌核的萌发,抑制率达60%~70%;在油菜离体叶片试验中,BY-2对菌核病的防治效果达100%。
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| 关 键 词： | 油菜 内生细菌 油菜体内定殖 菌核病菌 对峙培养 |
| 文章编号： | 0412-0914(2007)02-0192-05 |
| 修稿时间： | 2005年9月12日 |
Colonization of antifungal endobacterium BY-2 in oilcrop rape and its control effect on disease caused by Sclerotinia sclerotiorum |
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| JIANG Mu-lan,ZHAO Rui,HU Xiao-jia,ZHANG Yin-bo,WANG guo-ping.Colonization of antifungal endobacterium BY-2 in oilcrop rape and its control effect on disease caused by Sclerotinia sclerotiorum[J].Acta Phytopathologica Sinica,2007,37(2):192-196. |
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| Authors: | JIANG Mu-lan ZHAO Rui HU Xiao-jia ZHANG Yin-bo WANG guo-ping |
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| Institution: | 1 College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430062, China;2 Oil Crops Research Institute of CAAS, Wuhan 430062, China;3 Key Lab of Genetics Improvement for Oil Crops, Wuhan 430062, China |
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| Abstract: | Endophytic Bacillus subtilis BY-2 was isolated from oilseed rape. After return inoculation treat-ment to oilseed rape, the same endophytic B. subtilis as BY-2 was obtained. In 10 d after inoculation, the number of endophytic bacterium reached up to (2.24-9.02)×103 cfu/g fresh plant. 25 d later, the number of bacterium was maintained (3.13-8.59)×103 cfu/g fresh plant. The control effect of BY-2 on Sclerotinia sclerotiorum was investigated. After 2-day-confronting incubation on PDA plates, the inhibition zone reached to 3.1 cm. Meantime, serious mycelium malformation of S. sclerotiorum caused by BY-2 was observed, such as short growth, cytoplasm condensation, cell wall break and protoplasm leak. Even germination of sclerotia was also inhibited, the inhibition rate reached to 60%-70%. The control efficacy of BY-2 against S. sclero-tiorum was 100% on detached leaves in vitro. |
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| Keywords: | oilseed rape endophyte bacterium colonization trend Sclerotinia sclerotiorum confronting incubation |
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