| 根癌家杆菌介导的玉米遗传转化体系的建立 |
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| 引用本文： | 张荣,王国英,张晓红,赵虎基.根癌家杆菌介导的玉米遗传转化体系的建立[J].农业生物技术学报,2001,9(1):45-48. |
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| 作者姓名： | 张荣 王国英 张晓红 赵虎基 |
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| 作者单位： | 中国农业大学 |
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| 基金项目： | 国家“863”项目“转基因植物专项”基金资助 |
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| 摘 要： | 用根癌农杆菌菌株LBA4404（pTOK233)转化多种基因型的玉米幼胚。幼胚与农杆菌共培养3d后，检测到了GUS基因的瞬时表达。利用GUS基因的瞬时表达对农杆菌转化玉米的条件进行了优化，共培养后的幼不经含潮霉素的培养基筛选培养两个月后，获得了多种基因型的抗性愈伤组织。其中综3自交系的抗性愈伤组织分化出植株，P9－10、综3自交系R0结实得到后代。Southern杂交分析证实了霉素基因在玉米自交系P9－10基因组中的整合，建立了农杆菌介导的玉米遗传转化体系。
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| 关 键 词： | 玉米 根癌农杆菌 遗传转化 |
| 修稿时间： | 2000年5月23日 |
Agrobacterium tumefaciens Mediated Maize Transformation |
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| Zhang Rong,Wang Guoying,Zhang Xiaohong,Zhao Huji.Agrobacterium tumefaciens Mediated Maize Transformation[J].Journal of Agricultural Biotechnology,2001,9(1):45-48. |
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| Authors: | Zhang Rong Wang Guoying Zhang Xiaohong Zhao Huji |
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| Abstract: | Maize immature embryos of several genotype were transformed by Agrobacterium tumefaciens LBA4404(pTOK233),Factors influencing maize transformation mediated by Agrobacteriam tumefaciens have been studied through GUS transient expression. After 3 days of co-cultivation,blue spots were observed on immature embryos.Several genotype Resistant calli of several genotypes were obtained through two months of selection on N6 medium containing 20 mg/L hygromycin.The resistant calli of inbred P9-10、Z3 and F1 hybrid Z31 regenerated plants.Almost all regenerated plants were normal in morphology and fertile.R1 of inbred P9-10、Z3 were gained.The results of southern analysis of R0 transformants of inbred P9-10 indicated that the HPT gene has been inserted into maize genome. |
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| Keywords: | maize Agrobacterium tumefaciens transformation |
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