Assessment of Detoxifying Markers for Florfenicol in Rainbow Trout Liver |
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| Authors: | Antonia Concetta Elia Nicole Pacini Maria Letizia Fioravanti Ambrosius Josef Martin Dörr Annalisa Zaccaroni Alba Maria Parmeggiani |
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| Institution: | 1. Department of Chemistry, Biology and Biotechnology, University of Perugia, Via Elce di Sotto, 8,Perugia 06123, Italy;2. Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di Sopra, 50,Ozzano Emilia 40064, Italy |
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| Abstract: | Florfenicol (FF) is employed in fish farms to contest or prevent bacterial infections. However, this pharmaceutical may produce reactive oxygen species that may cause biochemical changes in antibiotic-treated fish. The aim of this study was to evaluate the effect of FF on Rainbow Trout Oncorhynchus mykiss treated for 10 d with 7.5 and 15 mg/kg FF followed by a withdrawal period of 5 d. Superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, glutathione S-transferase, glyoxalase I and glyoxalase II, total glutathione, lactic dehydrogenase, and alkaline phosphatase were investigated in the livers of treated and untreated fish. A general impairment of antioxidant enzymes and metabolic indicators was measured in FF-treated Rainbow Trout. Onset of oxidative damage may have occurred during the antibiotic treatment as a consequence of the effect of FF toxicity at mainly the highest dose. Nevertheless, the rise in levels of total glutathione and glutathione S-transferase even after the withdrawal period may shield the antibiotic-mediated oxidative processes. Received December 22, 2015; accepted May 26, 2016 Published online October 28, 2016 |
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