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不结球白菜同源异型突变雄性不育系HGMS的发现和初步研究
引用本文:张彦锋,王学芳,张新,李殿荣,梁宗锁.不结球白菜同源异型突变雄性不育系HGMS的发现和初步研究[J].西北农业学报,2005,14(6):164-168.
作者姓名:张彦锋  王学芳  张新  李殿荣  梁宗锁
作者单位:1. 西北农林科技大学生命科学学院,陕西,杨凌,712100;陕西省杂交油菜研究中心,大荔,许庄,715105
2. 陕西省杂交油菜研究中心,大荔,许庄,715105
3. 西北农林科技大学生命科学学院,陕西,杨凌,712100
摘    要:从不结球白菜高代自交系中发现并育成不结球白菜同源异型突变细胞核雄性不育系HGMS(homeotic GMS).不育特征为心皮在雄蕊位置错位表达,雄蕊消失,雌雄两性花变成无雄蕊单性雌花,心皮化的雄蕊上着生有胚珠,是一种全新的雄性不育.该不育系的花瓣严重退化,长、宽变短,重量减轻;雌蕊由细长变为短粗、重量增加.进一步同工酶研究发现,雄蕊位心皮的酯酶酶带和过氧化物酶酶带与本身雌蕊酶带一致,充分表明该雄性不育确系心皮异位表达引起.并根据花发育的ABC模型推测,该突变很可能是花发育的B组基因缺失所致.

关 键 词:不结球白菜  雄蕊心皮化  细胞核雄性不育  同源异型
文章编号:1004-1389(2005)06-0164-05
收稿时间:2005/8/11 0:00:00
修稿时间:2005年8月11日

The Discovery and Study on Homeotic Mutant Male Sterile Line HGMS of Nonheading Chinese-cabbage
ZHANG Yan-feng,WANG Xue-fang,ZHANG Xin,LI Dian-rong and LIANG Zong-suo.The Discovery and Study on Homeotic Mutant Male Sterile Line HGMS of Nonheading Chinese-cabbage[J].Acta Agriculturae Boreali-occidentalis Sinica,2005,14(6):164-168.
Authors:ZHANG Yan-feng  WANG Xue-fang  ZHANG Xin  LI Dian-rong and LIANG Zong-suo
Institution:College of Life Sciences, Northwest A & F University, Yangling Shaanxi 712100, China;Hybrid Rapeseed Research Center of Shaanxi Province, Dali Shaanxi 715105, China;Hybrid Rapeseed Research Center of Shaanxi Province, Dali Shaanxi 715105, China;Hybrid Rapeseed Research Center of Shaanxi Province, Dali Shaanxi 715105, China;Hybrid Rapeseed Research Center of Shaanxi Province, Dali Shaanxi 715105, China;College of Life Sciences, Northwest A & F University, Yangling Shaanxi 712100, China
Abstract:Homeotic mutation male sterile material was discovered from the high generation selfing materials of nonheading Chinese-cabbage and homeotic Gms (HGMS) was bred. HGMS is a new type of GMS, which sterile identity appeared as ectopic expression of carpel on stamen, and stamen vanished, and bisexual flower changed into unisexual flower without stamen and its carpeloid stamen with some ovules. The sterile line's petals degenerate obviously, and their length and width shortened, weight lightened. And its pistil changed from slim to thickset, and weight increased. The result of isoenzyme analysis also showed that band of esterase and POD of carpeloid organ were consistent with itself pistil's. It is indicated that the mutation sterile identity was caused by carpel ectopic expression. According to ABC model of flower development, it was supposed that the mutantion is caused possibly by loss of B gene.
Keywords:Nonheading Chinese-cabbage  Carpeloid stamen  GMS  Homeotic
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