| 小麦成熟胚的组织培养 |
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| 作者姓名： | 毕瑞明 王洪刚 |
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| 作者单位： | 菏泽学院生命科学系，国家小麦改良中心泰安分中心作物生物学国家重点实验室山东农业大学农学院 菏泽274015 国家小麦改良中心泰安分中心作物生物学国家重点实验室山东农业大学农学院，泰安271018，泰安271018 |
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| 基金项目： | 国家农业部农业结构调整重大技术研究专项（04-02-05B）资助项目. |
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| 摘 要： | 【研究目的】为了筛选出小麦(TriticumaestivumL.)成熟胚(MEs)培养和遗传转化受体材料的优良基因型,【方法】以27份优良普通小麦冬性主栽品种或高代育种品系为试材,采用整粒切胚诱愈方法(endosperm-supportedmethod),对小麦成熟胚进行组织培养研究。【结果】结果表明,在成熟胚培养过程中光照、温度、培养基2,4-D浓度、基因型等因素对小麦成熟胚组织培养影响较大;同时小麦成熟胚组织培养存在着较强的基因型依赖性,不同基因型愈伤组织诱导率、分化率、成苗率的差异具有统计学意义(P<0.01)。通过研究从27个小麦基因型中筛选出了3个适合于成熟胚整粒切胚诱愈的基因型,即山农2618、泰山021、河北341,他们的成苗率分别达到了15.38%、25.27%、21.33%。【结论】小麦成熟胚组织培养优良基因型的筛选,为以后小麦的遗传转化和应用基因工程技术进行遗传改良奠定了基础。
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| 关 键 词： | 小麦 成熟胚 组织培养 |
Study on Tissue Culture of Mature Embryo from Wheat |
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| Authors: | Bi Ruiming Wang Honggang |
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| Abstract: | OBJECTIVE]In order to select out the optimum wheat (Triticum aestivum L.) genotype for tissue culture of mature embryo (MEs) and receptor of genetic transformation, METHOD]27 winter-type superior cultivars wheat of Chinese northern region or breeding lines of hexaploid common wheat were used as test material and the method of callus induction of endosperm-supported was utilized in this paper. The effect of many factors on tissue culture of wheat MEs had been studied. RESULTS]The results indicated that, light, temperature, concentration of 2,4-D and genotype were important influence factors for tissue culture of wheat MEs. The genotype dependency was strongly high in tissue culture of Mes of these genotypes. Differences of frequency of induction, differentiation and regeneration of callus from mature embryos of 27 wheat genotypes by endosperm-supported method were respectively significant (P<0.01). The efficiency of induction, differentiation and regeneration of mature embryos callus was high in genotypes screened out. Mature embryo-derived callus of SN2618, TS021, HB341 showed better tissue culture response than other genotypes. Their frequency of regeneration were 15.38%, 25.27%, 21.33% respectively. The regenerated plants could be obtained in 30~40 d. CONCLUSION]The work provided a solid basis for tissue culture of wheat Mes, genetic transformation and molecular plant breeding of wheat. |
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| Keywords: | Wheat Mature embryo Tissue culture |
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