| 羊草乙醛脱氢酶（ALDH）基因片段的克隆及表达分析 |
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| 作者姓名： | 李新玲 杨传平 徐香玲 |
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| 作者单位： | 哈尔滨师范大学生命与环境科学学院，东北林业大学林木遗传育种研究室，哈尔滨师范大学生命与环境科学学院 哈尔滨150025东北林业大学林木遗传育种研究室，哈尔滨150040，哈尔滨150040，哈尔滨150025 |
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| 基金项目： | 黑龙江省重大攻关课题（GA068103-10）;哈尔滨市培养学科后备带头人基金（2005AFXXJ027） |
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| 摘 要： | 研究目的]克隆羊草的乙醛脱氢酶ALDH基因片段,研究该基因在不同条件下的表达情况。方法]采用RT-PCR技术克隆羊草的ALDH基因片段,并对其核苷酸和氨基酸序列进行分析,采用RealTimeRT-PCR方法研究该基因的表达。结果]获得了羊草的ALDH基因片段,长度为675bp,编码225aa。核苷酸序列比较表明,与水稻ALDH1a序列(AB037421)同源性为86%,与玉米RF2C(AF348413)同源性为85%。BLASTp分析,该序列与水稻、玉米、拟南芥的乙醛脱氢酶一致性分别高达87%、86%、60%,含有醛脱氢酶基因家族的保守结构域。RealTimeRT-PCR数据表明,在诱导条件下该基因的表达量呈先升高后降低的趋势。总体上来说,该基因对盐的响应要高于干旱和冷冻。结论]成功获得了羊草ALDH基因片段,并研究了该基因的表达情况,为进一步克隆羊草ALDH全长基因奠定了基础。
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| 关 键 词： | 羊草 乙醛脱氢酶基因 RealTime RT-PCR |
Molecular Cloning and Expression Analysis of Aldehyde Dehydrogenase (ALDH) Gene Segment in Leymus Chinensis |
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| Authors: | Li Xinling Yang Chuanping Xu Xiangling |
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| Institution: | 1 School of Life and Environmental Science, Harbin Normal University, Harbin 1500251 2 Teaching and Research Section of Forestry Genetics and Breeding Northeast Forestry University, Harbin 150040 |
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| Abstract: | Objective]Aldehyde Dehydrogenase (ALDH) gene segment from Leymus Chinensis was cloned and the expression of it was researched under the different situations.Method]ALDH gene segment was cloned by RT-PCR. The nucleotide and amino acid sequence of ALDH were analyzed. The expression of ALDH was researched by Real Time RT-PCR.Result]ALDH gene segment was obtained which was 675bp and coding 225aa. The compare of nucleotide sequences indicated that it has 86% identity with rice ALDH1a (AB037421), and 85% identity with maize RF2C(AF348413). By BLASTp Analysis, it shares 87%, 86% and 60% amino acid sequence identity with rice, maize and Arabidopsis thaliana ALDH, respectively, which containing conservation region of aldehyde dehydrogenase families. The data of Real Time RT-PCR indicated that the expression of ALDH was increased first, and then decreased under the inducing situations. As a whole, the responses of ALDH to salt and drought were much higher than to cold.Conclusion]In this research, ALDH gene segment was obtained, and we researched the expression of ALDH. It established basis on cloning the span of ALDH in Leymus Chinensis. |
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| Keywords: | Leymus Chinensis aldehyde dehydrogenase Real Time RT-PCR |
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